Arginine inhibits Saccharomyces cerevisiae biofilm formation by inducing endocytosis of the arginine transporter Can1
Arginine inhibits Saccharomyces cerevisiae biofilm formation by inducing endocytosis of the arginine transporter Can1
复制标题
精氨酸通过诱导精氨酸转运蛋白 Can1 的内吞作用抑制酿酒酵母生物膜形成
DOI：
10.1093/bbb/zbac094
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Takagi Hiroshi
中科院分区：
文献类型：
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作者：
Nishimura Akira;Nakagami Kazuki;Kan Kyoyuki;Morita Fumika;Takagi Hiroshi
生物膜是由微生物聚集成粘附于表面的多细胞结构而形成的。由于酵母菌生物膜对非生物表面和组织的强粘附性，酵母菌生物膜的形成是临床和工业领域的关键问题。在这里，我们澄清了由酵母菌介导的生物膜形成的抑制。首先，我们发现精氨酸可以抑制酵母菌、光滑念珠菌和枝孢霉等真菌生物膜的形成，但对细菌没有影响。关于潜在的机制，生化分析表明，精氨酸通过抑制Flo11依赖性絮凝来抑制生物膜形成。有趣的是，缺失精氨酸转运蛋白编码CAN1的菌株对精氨酸介导的生物膜形成抑制不敏感。最后，Can1内吞作用似乎是精氨酸抑制生物膜形成机制所必需的。本研究结果有助于阐明酵母生物膜形成及其调控的分子机制。
Biofilms are formed by the aggregation of microorganisms into multicellular structures that adhere to surfaces. Biofilm formation by yeast is a critical issue in clinical and industrial fields because of the strong adhesion of yeast biofilm to abiotic surfaces and tissues. Here, we clarified the arginine-mediated inhibition of biofilm formation by yeast. First, we showed that arginine inhibits biofilm formation in fungi such asSaccharomyces cerevisiae, Candida glabrata, andCladosporium cladosporioides, but not in bacteria. In regard to the underlying mechanism, biochemical analysis indicated that arginine inhibits biofilm formation by suppressing Flo11-dependent flocculation. Intriguingly, a strain with deletion of the arginine transporter-encodingCAN1was insensitive to arginine-mediated inhibition of biofilm formation. Finally, Can1 endocytosis appeared to be required for the inhibitory mechanism of biofilm formation by arginine. The present results could help to elucidate the molecular mechanism of yeast biofilm formation and its control.