Wheat germ agglutinin-modified trifunctional nanospheres for cell recognition.
Wheat germ agglutinin-modified trifunctional nanospheres for cell recognition.
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DOI：
10.1021/bc060387g
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发表时间：
2007-09
影响因子：
4.7
通讯作者：
Hai‐Yan Xie;Min Xie;Zhiling Zhang;Yan-Min Long;Xin Liu;Ming-liang Tang;D. Pang;Zheng Tan;C. Dickinson;Wuzong Zhou
中科院分区：
文献类型：
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作者：
Hai‐Yan Xie;Min Xie;Zhiling Zhang;Yan-Min Long;Xin Liu;Ming-liang Tang;D. Pang;Zheng Tan;C. Dickinson;Wuzong Zhou
提出了一种简便的方法，制备了识别人前列腺癌DU-145细胞表面表达唾液酸和N-乙酰氨基葡萄糖的智能荧光磁性麦胚凝集素修饰三功能纳米球（WGA-TFNS）。这些TFNS可以容易地操作、跟踪，并且方便地用于捕获和分离靶细胞。通过红外光谱、羧甲基甲壳素修饰量子点（CM-CT-QDs）的生物识别和金黄色葡萄球菌（Staphylococcus aureus）的生物识别，证实了麦胚凝集素在WGA-TFNS表面的存在。WGA-TFNS识别DU-145细胞的成功表明WGA-TFNS是适用的。
A simple and convenient strategy has been put forward to fabricate smart fluorescent magnetic wheat germ agglutinin-modified trifunctional nanospheres (WGA-TFNS) for recognition of human prostate carcinoma DU-145 cells which are surface-expressed with sialic acid and N-acetylglucosamine. These TFNS can be easily manipulated, tracked, and conveniently used to capture and separate target cells. The presence of wheat germ agglutinin on the surface of WGA-TFNS was confirmed by FTIR, biorecognition of carboxymethyl chitin-modified quantum dots (CM-CT-QDs), and bacterium Staphylococcus aureus. The success in recognizing DU-145 cells by the WGA-TFNS indicates that WGA-TFNS could be applicable.