Tissue- and species-specific antigens of normal human prostatic tissue.
Tissue- and species-specific antigens of normal human prostatic tissue.
复制标题
正常人前列腺组织的组织和物种特异性抗原。
作者：
R. Ablin;P. Bronson;W. A. Soanes;E. Witebsky
用含有完全弗氏佐剂的正常人前列腺组织的盐水提取物对家兔进行异种免疫，产生的抗体与正常人前列腺组织的盐水提取物以及其他人体组织和液体的类似提取物制剂反应，这是通过双扩散凝胶沉淀和鞣革细胞血凝标准判断的。兔抗血清对正常人前列腺组织提取物的严格吸收研究，伴随着凝胶扩散板在含有硝酸铅和β-甘油磷酸钠的缓冲液-底物混合物中孵育后沉淀线分辨率的提高，表明这些抗体中的一部分是前列腺特异性的，此外还允许鉴定两种前列腺组织特异性抗原。通过对凝胶沉淀模式的特异性免疫化学染色，将前列腺组织特异性抗原之一鉴定为前列腺酸性磷酸酶。
正常人前列腺抗原的种属特异性研究表明，犬和兔前列腺组织的制剂中不存在类似的抗原，但显然在一定程度上与猴前列腺的颅叶抗原共享。
前列腺良性和恶性疾病患者的几个血清沉淀反应伴随着前列腺酸性磷酸酶染色，但这种反应的相关性仍有待确定。此外，初步凝胶扩散沉淀研究表明正常、良性和恶性前列腺组织提取物之间存在特定的抗原差异。
正常人前列腺组织提取物和特异性吸收的同种抗血清的免疫电泳分析显示，在电泳场的βγ区有一个沉淀弧。通过在缓冲液-底物混合物中孵育和酸性磷酸酶染色获得的免疫电泳图谱的进一步分辨率揭示了两到三条额外的沉淀线，沉淀的主要弧被鉴定为前列腺酸性磷酸酶。
通过凝胶扩散沉淀和免疫电泳评价兔抗血清对正常人前列腺组织提取物的敏感性和特异性，以及这种免疫反应的发展，通过直接鞣革细胞血凝和鞣革细胞血凝抑制证实。
Heteroimmunization of rabbits with saline extracts of normal human prostatic tissue incorporating complete Freund's adjuvant produced antibodies reacting with saline extracts of normal human prostatic tissue as well as with comparable extract preparations of other human tissues and fluids as judged by the criteria of double diffusion gel precipitation and tanned cell hemagglutination. Rigorous absorption studies of rabbit antisera to extracts of normal human prostatic tissue accompanied by enhanced resolution of lines of precipitation following incubation of gel diffusion plates in a buffer-substrate mixture incorporating lead nitrate and sodium-β-glycerophosphate revealed that a portion of these antibodies were prostate-specific, and in addition permitted identification of two prostatic tissue-specific antigens. By specific immunochemical staining of the gel precipitation pattern, one of the prostate tissue-specific antigens was identified as prostatic acid phosphatase.
Species-specificity studies of the antigens of the normal human prostate revealed that comparable antigens were absent from preparations of canine and rabbit prostatic tissue, but apparently shared to some degree with antigens of the cranial lobe of the monkey prostate.
Several sera of patients with benign and malignant diseases of the prostate gave precipitation reactions accompanied by staining for prostatic acid phosphatase, but the relevance of such reactions remains to be determined. In addition, preliminary gel diffusion precipitation studies suggested specific antigenic differences between normal, benign and malignant prostatic tissue extracts.
Immunoelectrophoretic analysis of extracts of normal human prostatic tissue and specifically absorbed homologous antisera revealed one arc of precipitation in the βγ-region of the electrophoretic field. Further resolution of immunoelectrophoretic patterns obtained by incubation in the buffer-substrate mixture and staining for acid phosphatase disclosed two to three additional lines of precipitation, the major arc of precipitation being identified as prostatic acid phosphatase.
The sensitivity and specificity of rabbit antisera to extracts of normal human prostatic tissue as evaluated by gel diffusion precipitation and immunoelectrophoresis, as well as the development of this immunologic response, was confirmed by both direct tanned cell hemagglutination and inhibition of tanned cell hemagglutination.