Calmodulin- and Ca2+-Dependent Facilitation and Inactivation of the CaV1.2 Ca2+ Channels in Guinea-Pig Ventricular Myocytes
Calmodulin- and Ca2+-Dependent Facilitation and Inactivation of the CaV1.2 Ca2+ Channels in Guinea-Pig Ventricular Myocytes
复制标题
豚鼠心室肌细胞中 CaV1.2 Ca2 通道的钙调蛋白和 Ca2 依赖性促进和失活
DOI：
10.1254/jphs.09282fp
复制
发表时间：
2010-03-01
影响因子：
3.5
通讯作者：
Kameyama, Masaki
中科院分区：
文献类型：
--
作者：
Han, Dong-Yun;Minobe, Etsuko;Kameyama, Masaki
L型钙通道（Ca（V）1.2）显示明显的钙依赖性易化和失活。本文在豚鼠心室肌细胞内面向外膜片模式下，观察了钙调素（CaM）和Ca ~（2+）对Ca ~（2+）通道的影响。在游离[Ca 2 +]为0.1 μ M时，CaM（0.15、0.7、1.4、2.1、3.5和7.0 μ M）+ ATP（2.4 mM）诱导的通道活性分别为对照活性的27%、98%、142%、222%、65%和20%，显示出钟形关系。在游离[Ca 2 +]
The L-type Ca2+ channel (Ca(V)1.2) shows clear Ca2+-dependent facilitation and inactivation. Here we have examined the effects of calmodulin (CaM) and Ca2+ on Ca2+ channel in guinea-pig ventricular myocytes in the inside-out patch mode, where rundown of the channels was controlled. At a free [Ca2+] of 0.1 mu M, CaM (0.15, 0.7, 1.4, 2.1, 3.5, and 7.0 mu M) + ATP (2.4 mM) induced channel activities of 27%, 98%, 142%, 222%, 65%, and 20% relative to the control activity, respectively, showing a bell-shaped relationship. Similar results were observed at a free [Ca2+]