Expression of gelatinases A and B, and TIMPs 1 and 2 during corneal wound healing.
Expression of gelatinases A and B, and TIMPs 1 and 2 during corneal wound healing.
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DOI：
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发表时间：
1998-05
影响因子：
4.4
通讯作者：
H. Ye;Dimitri T. Azar
中科院分区：
文献类型：
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作者：
H. Ye;Dimitri T. Azar
目的观察大鼠角膜创伤愈合早期明胶酶A、B的表达及基质金属蛋白酶组织抑制剂（TIMP）-1、TIMP-2的定位。方法. 30只成年Sprague-Dawley大鼠的一只眼睛接受准分子激光角膜切除术治疗，另一只眼睛未接受治疗。伤后6，18，24小时和3，7天获得的角膜冷冻切片用于进行免疫共聚焦显微镜和原位杂交。结果明胶酶B在伤后18、24 h和3 d免疫组化定位于基底膜区和浅表基质，其mRNA仅定位于基底上皮细胞。基质金属蛋白酶组织抑制剂-1也在同一时间点免疫定位于基底膜区和浅表基质。明胶酶A免疫定位于正常角膜的上皮和基质，并在伤后3天和7天主要分布于基底上皮和浅表基质。原位杂交证实了上皮细胞和基质角膜细胞表达明胶酶A。金属蛋白酶组织抑制剂-2在正常和受伤的角膜上皮免疫定位。伤后3天，基底膜区可见强烈的TIMP-2标记。结论：与明胶酶A不同，明胶酶B仅在创伤后迁移的基底上皮细胞中表达。基质金属蛋白酶-金属蛋白酶组织抑制剂（MMP/TIMP）系统在准分子激光角膜切削术后角膜创面愈合的早期可能起重要作用。
PURPOSE To determine the expression of gelatinases A and B and the localization of tissue inhibitors of metalloproteinase (TIMP)-1 and TIMP-2 during the early stages of corneal wound healing in rats. METHODS. One eye each of 30 adult Sprague-Dawley rats was treated with excimer laser keratectomy; the other eye was untreated. Frozen sections of corneas obtained 6, 18, and 24 hours and 3 and 7 days after wounding were used to perform immunoconfocal microscopy and in situ hybridization. RESULTS Gelatinase B was immunolocalized to the basement membrane zone and superficial stroma, and its mRNA was exclusively localized to basal epithelial cells migrating across the wound at 18 and 24 hours and 3 days after wounding. Tissue inhibitor of metalloproteinase-1 was also immunolocalized to the basement membrane zone and superficial stroma at the same time points. Gelatinase A was immunolocalized to the epithelium and stroma of normal corneas and was predominant in the basal epithelium and superficial stroma at 3 and 7 days after wounding. In situ hybridization confirmed gelatinase A expression by the epithelial cells and the stromal keratocytes. Tissue inhibitor of metalloproteinase-2 was immunolocalized to the epithelium in normal and wounded corneas. Intense TIMP-2 labeling of the basement membrane zone was noted 3 days after wounding. CONCLUSIONS Unlike gelatinase A, gelatinase B is expressed exclusively by migrating basal epithelial cells after wounding. The matrix metalloproteinase-tissue inhibitor of metalloproteinase (MMP)/TIMP systems may play an important role in the early stages of corneal wound healing after excimer laser keratectomy.