Modulation of the ecdysteroid-induced cell death by juvenile hormone during pupal wing development of lepidoptera
Modulation of the ecdysteroid-induced cell death by juvenile hormone during pupal wing development of lepidoptera
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DOI：
10.1002/arch.20192
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发表时间：
2007-07-01
影响因子：
2.2
通讯作者：
Fujiwara, Haruhiko
中科院分区：
文献类型：
--
作者：
Lobbia, Saori;Futahashi, Ryo;Fujiwara, Haruhiko
长缘毛蛾雌蛾只有退化的翅膀，而雄蛾有正常的翅膀。我们先前发现蜕皮激素以性别和区域特异性的方式诱导细胞凋亡和吞噬激活。为了研究蜕皮类固醇的不同反应是否在受体水平上受到控制，我们克隆了蜕皮类固醇受体亚型ECR-A和ECR-B1。在雄翼和雌翼中，ECR-A信号出现在交界陷窝的远侧区域，而ECR-B1信号出现在交界陷窝的近侧区域。这两种ECR亚型的表达模式相似，表明ECR以外的其他分子可能参与了雄性和雌性之间不同的蜕皮激素反应。接下来，我们测试了保幼激素(JH)对二化螟蛹翅形态发生的影响。有趣的是，JH和20E的添加不仅在雌性中诱导了翅的退化，而且在雄性中也引起了翅的退化。此外，较高浓度的JH对家蚕的蛹翅进行预处理，在蜕皮激素处理下也会引起家蚕翅的退化。这些结果表明，JH调节蜕皮激素的作用，诱导鳞翅目中普遍存在的蛹翅上的细胞死亡。
Females of the tussock moth Orgyia recens have only vestigial wings, whereas the males have normal wings. We previously found that ecdysteroid induces both apoptotic events and phagocytotic activation in sex-specific and region-specific manners. To investigate whether different responses to ecdysteroid are controlled at the receptor level, we cloned ecdysteroid receptor isoforms, EcR-A and EcR-B1, in O. recens. In both male and female wings, EcR-A signal was detected in the distal region of the bordering lacuna (BL), whereas EcR-B1 signal was detected in the proximal region of the BL. The similar expression patterns of both EcR isoforms suggested that molecules other than EcR should be involved in different ecdysteroid responses between male and female of O. recens. We next tested juvenile hormone (JH) effects on pupal wing morphogenesis in O. recens. Interestingly, both JH and 20E addition induced wing degeneration not only in females but also in males. In addition, higher concentration of JH pre-treatment of the pupal wings of the silkworm, Bombyx mori, also caused wing degeneration under ecdysteroid treatment. These results indicate that JH modulates the ecdysteroid action to induce the cell death on pupal wings, generally in Lepidoptera.