FACTORS INFLUENCING EFFLUX OF [H-3] GAMMA-AMINOBUTYRIC ACID FROM SATELLITE GLIAL CELLS IN RAT SENSORY GANGLIA
FACTORS INFLUENCING EFFLUX OF [H-3] GAMMA-AMINOBUTYRIC ACID FROM SATELLITE GLIAL CELLS IN RAT SENSORY GANGLIA
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DOI：
10.1111/j.1471-4159.1975.tb07676.x
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发表时间：
1975-01-01
影响因子：
4.7
通讯作者：
MINCHIN, MCW
中科院分区：
文献类型：
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作者：
MINCHIN, MCW
在10‐5M‐氨基氧乙酸存在的情况下，研究了大鼠背根神经节卫星胶质细胞自发流出[3H]GABA和64 mM‐K+引起的流出，发现不受10‐4M‐D 600或在没有Mg2+的情况下升高的（9.6mM） Ca2+的影响。[3H]用胆碱离子代替洗涤介质中的钠离子增加GABA的流出，而64 mM‐K+不能进一步增加流出。缬曲定（10‐6和10‐4M）和蛇曲霉毒素（10‐8和10‐6M）不能改变神经胶质细胞自发流出[3H]GABA。我们测试了多种化合物，包括氨基酸、GABA类似物和GABA拮抗剂对[3H]GABA外排的影响。结果表明，抑制GABA进入神经胶质细胞的化合物也能够刺激这些细胞的[3H]GABA外排。这些结果与神经胶质细胞释放GABA的可能机制进行了讨论。
The spontaneous efflux of [3H]GABA from the satellite glial cells of rat dorsal root ganglia and the efflux evoked by 64 mM‐K+were studied in the presence of 10‐5M‐amino‐oxyacetic acid and found not to be affected by 10‐4M‐D 600 or by elevated (9.6mM) Ca2+in the absence of Mg2+. [3H]GABA efflux was increased by replacing sodium ions in the washing medium by choline ions and 64 mM‐K+failed to increase the efflux further. The drugs veratridine (10‐6and 10‐4M) and batrachotoxin (10‐8and 10‐6M) failed to alter the spontaneous efflux of [3H]GABA from the glial cells.A variety of compounds, including amino acids, a GABA analogue and a GABA antagonist were tested for their ability to affect [3H]GABA efflux. The results indicated that compounds which inhibit GABA uptake into glial cells were also able to stimulate [3H]GABA efflux from these cells.The results are discussed with reference to possible mechanisms involved in the release of GABA from glial cells.