Analysis of desiccation and vitrification characteristics of carbohydrate films by shear-wave resonators
Analysis of desiccation and vitrification characteristics of carbohydrate films by shear-wave resonators
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DOI：
10.1021/la047760y
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发表时间：
2005-03-29
期刊：
影响因子：
3.9
通讯作者：
Toner, M
中科院分区：
文献类型：
--
作者：
Aksan, A;Morris, SC;Toner, M
在过去的二十年里，在碳水化合物存在下哺乳动物细胞和组织的干燥状态保存已经开始显示出希望。某些碳水化合物通过降低干燥状态下的分子流动性而在保存中起主要作用。在这次交流中，利用剪切波谐振器收集实时分子迁移率信息的碳水化合物基薄膜的干燥和玻璃化过程中的可行性进行了论证。同时石英晶体微天平实验和光学成像被用来确定薄膜形成的条件和某些碳水化合物的生物重要性的解决方案的玻璃化动力学。使用这里提出的技术，有可能深入了解碳水化合物溶液的玻璃化特性，为未来的研究奠定基础，定量分析薄膜特性和活哺乳动物细胞实验。
Desiccated state preservation of mammalian cells and tissues in the presence of carbohydrates has started to show promise in the last two decades. Certain carbohydrates play a major role in preservation by reducing molecular mobility in the desiccated state. In this communication, the feasibility of utilizing shear-wave resonators to collect real-time molecular mobility information during desiccation and vitrification of carbohydrate based thin films was demonstrated. Simultaneous quartz crystal microbalance experimentation and optical imaging were utilized to determine the conditions for thin film formation and the vitrification kinetics of certain carbohydrate solutions of biological importance. Using the technique presented here, it was possible to gain insight into the vitrification characteristics of carbohydrate solutions establishing the basics for future research with quantitative analysis of film properties and experimentation with live mammalian cells.