Establishing a small animal model for evaluating protective immunity against mumps virus.
Establishing a small animal model for evaluating protective immunity against mumps virus.
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DOI：
10.1371/journal.pone.0174444
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
He B
中科院分区：
文献类型：
--
作者：
Pickar A;Xu P;Elson A;Zengel J;Sauder C;Rubin S;He B
尽管流行性腮腺炎疫苗已经使用了几十年，但保护性免疫相关因素还没有被定义。最近，在接种疫苗的人群中发生了流行性腮腺炎疫情。为了更好地了解暴发的原因并开发控制流行性腮腺炎疫苗免疫人群暴发的方法，定义保护性免疫相关因素将是至关重要的。不幸的是，目前还没有评估腮腺炎免疫力的小动物模型。在这项研究中，我们评估了I型干扰素α/β受体基因敲除小鼠(干扰素-α/βR−/−)在此类模型中的应用。我们发现这些小鼠对腮腺炎病毒易感，无论是鼻腔内还是脑内注射。被动转移免疫小鼠纯化的免疫球蛋白可保护幼龄小鼠免受腮腺炎病毒感染，证实了抗体的保护作用，并显示了该模型用于评价腮腺炎免疫的潜力。
Although mumps vaccines have been used for several decades, protective immune correlates have not been defined. Recently, mumps outbreaks have occurred in vaccinated populations. To better understand the causes of the outbreaks and to develop means to control outbreaks in mumps vaccine immunized populations, defining protective immune correlates will be critical. Unfortunately, no small animal model for assessing mumps immunity exists. In this study, we evaluated use of type I interferon (IFN) alpha/beta receptor knockout mice (IFN-α/βR−/−) for such a model. We found these mice to be susceptible to mumps virus administered intranasally and intracranially. Passive transfer of purified IgG from immunized mice protected naïve mice from mumps virus infection, confirming the role of antibody in protection and demonstrating the potential for this model to evaluate mumps immunity.