The Impact of Altered Gravity and Vibration on Endothelial Cells During a Parabolic Flight
The Impact of Altered Gravity and Vibration on Endothelial Cells During a Parabolic Flight
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DOI：
10.1159/000343380
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发表时间：
2013-01-01
影响因子：
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通讯作者：
Grimm, Daniela
中科院分区：
文献类型：
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作者：
Wehland, Markus;Ma, Xiao;Grimm, Daniela
背景：在改变重力条件下培养的内皮细胞 (EC) 显示细胞骨架紊乱和差异基因表达（短期影响），以及贴壁生长的 EC 细胞凋亡或管状 3D 结构的形成（长期影响）。方法：为了研究真实微重力的短期影响，我们将 EC 暴露于抛物线飞行动作，并在蛋白质和转录水平上进行分析。超重力和振动的影响分别进行了研究。结果：泛肌动蛋白和微管蛋白因振动而升高，因超重力而下调。 β-肌动蛋白因振动而减少。 Moesin 蛋白因振动和超重力而减少，埃兹蛋白在振动下强烈升高。 ACTB、CCND1、CDC6、CDKN1A、VEGFA、FLK-1、EZR、ITBG1、OPN、CASP3、CASP8、ANXA2 和 BIRC5 的基因表达在振动下降低。除 CCNA2、CCND1、MSN、RDX、OPN、BIRC5 和 ACTB 之外，所有研究的基因均因超重力而下调。一条抛物线 (P) 后，CCNA2、CCND1、CDC6、CDKN1A、EZR、MSN、OPN、VEGFA、CASP3、CASP8、ANXA1、ANXA2 和 BIRC5 上调，而 FLK1 下调。 EZR、MSN、OPN、ANXA2 和 BIRC5 在 31P 后表达上调。结论：抛物线飞行动作影响细胞骨架、血管生成、细胞外基质、细胞凋亡和细胞周期调控的基因。我们证明微重力刺激比超重力/振动更强。版权所有 (c) 2013 S. Karger AG，巴塞尔
Background: Endothelial cells (EC) cultured under altered gravity conditions show a cytoskeletal disorganization and differential gene expression (short-term effects), as well as apoptosis in adherently growing EC or formation of tubular 3D structures (long-term effects). Methods: Investigating short-term effects of real microgravity, we exposed EC to parabolic flight maneuvers and analysed them on both protein and transcriptional level. The effects of hypergravity and vibration were studied separately. Results: Pan-actin and tubulin proteins were elevated by vibration and down-regulated by hypergravity. beta-Actin was reduced by vibration. Moesin protein was reduced by both vibration and hypergravity, ezrin potein was strongly elevated under vibration. Gene expression of ACTB, CCND1, CDC6, CDKN1A, VEGFA, FLK-1, EZR, ITBG1, OPN, CASP3, CASP8, ANXA2, and BIRC5 was reduced under vibration. With the exception of CCNA2, CCND1, MSN, RDX, OPN, BIRC5, and ACTB all investigated genes were downregulated by hypergravity. After one parabola (P) CCNA2, CCND1, CDC6, CDKN1A, EZR, MSN, OPN, VEGFA, CASP3, CASP8, ANXA1, ANXA2, and BIRC5 were up-, while FLK1 was downregulated. EZR, MSN, OPN, ANXA2, and BIRC5 were upregulated after 31P. Conclusions: Genes of the cytoskeleton, angiogenesis, extracellular matrix, apoptosis, and cell cycle regulation were affected by parabolic flight maneuvers. We show that the microgravity stimulus is stronger than hypergravity/vibration. Copyright (c) 2013 S. Karger AG, Basel