Liquid-EM goes viral - visualizing structure and dynamics.
Liquid-EM goes viral - visualizing structure and dynamics.
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Liquid-EM 迅速走红——结构和动力学可视化。
DOI：
10.1016/j.sbi.2022.102426
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发表时间：
2022
影响因子：
6.8
通讯作者：
Dressel-Dukes,MadelineJ
中科院分区：
文献类型：
--
作者：
Kelly,DeborahF;DiCecco,Liza-Anastasia;Jonaid,GM;Dearnaley,WilliamJ;Spilman,MichaelS;Gray,JenniferL;Dressel-Dukes,MadelineJ
液体电子显微镜（EM），室温相关的冷冻EM，是一个令人兴奋的新技术提供实时数据的动态反应在溶液中。在这里，我们解释了如何液体EM获得普及，近年来通过检查进行病毒组装和宿主-病原体相互作用的关键实验。我们描述了开发工作流程的标本制备，数据收集和计算过程，导致第一个高分辨率的病毒结构在液体环境中。同样重要的是，我们回顾了为什么液体电子断层扫描技术可能成为生物医学研究中的下一件大事，因为它能够在几秒钟内监测进入细胞的活病毒。两者合计，我们提出的想法，液体EM可以作为一个动态的补充，目前的冷冻EM方法，激发了“实时革命”的纳米级成像。
Liquid-electron microscopy (EM), the room temperature correlate to cryo-EM, is an exciting new technique delivering real-time data of dynamic reactions in solution. Here, we explain how liquid-EM gained popularity in recent years by examining key experiments conducted on viral assemblies and host–pathogen interactions. We describe developing workflows for specimen preparation, data collection, and computing processes that led to the first high-resolution virus structures in a liquid environment. Equally important, we review why liquid-electron tomography may become the next big thing in biomedical research due to its ability to monitor live viruses entering cells within seconds. Taken together, we pose the idea that liquid-EM can serve as a dynamic complement to current cryo-EM methods, inspiring the “real-time revolution” in nanoscale imaging.