Compensatory mutations reducing the fitness cost of plasmid carriage occur in plant rhizosphere communities.
Compensatory mutations reducing the fitness cost of plasmid carriage occur in plant rhizosphere communities.
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在植物根际群落中，质量降低质粒的适应性成本的补偿性突变。
DOI：
10.1093/femsec/fiad027
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发表时间：
2023-03-23
影响因子：
4.2
通讯作者：
Brockhurst, Michael A.
中科院分区：
文献类型：
--
作者：
Bird, Susannah M.;Ford, Samuel;Thompson, Catriona M. A.;Little, Richard;Hall, James P. J.;Jackson, Robert W.;Malone, Jacob;Harrison, Ellie;Brockhurst, Michael A.
关键词：
质粒通过在谱系之间转移重要的生态功能来驱动细菌进化创新，但获得质粒通常以宿主细胞的适应性为代价。补偿突变，改善质粒携带的成本，促进质粒维持在简化的实验室介质中跨越不同的质粒宿主协会。然而，这种补偿性进化是否可以发生在更复杂的群落中，这些群落栖息在进化路径可能受到更多限制的自然环境小生境中，目前尚不清楚。在这里，我们显示了大量的健身成本进行大型接合质粒pQBR 103在荧光假单胞菌SBW 25在植物根际。这种质粒健身成本可以改善补偿突变影响染色体的全球调控系统gacA/gacS，迅速出现在植物根际社区和专为质粒载体。这些发现扩展了我们对补偿性进化在质粒动力学中的重要性的理解，超越了简化的实验室培养基。补偿性突变有助于生活在其环境生态位中的复杂微生物群落中的细菌群体中的质粒存活。在植物根际发生补偿突变，降低质粒携带的成本。
Plasmids drive bacterial evolutionary innovation by transferring ecologically important functions between lineages, but acquiring a plasmid often comes at a fitness cost to the host cell. Compensatory mutations, which ameliorate the cost of plasmid carriage, promote plasmid maintenance in simplified laboratory media across diverse plasmid–host associations. Whether such compensatory evolution can occur in more complex communities inhabiting natural environmental niches where evolutionary paths may be more constrained is, however, unclear. Here, we show a substantial fitness cost of carrying the large conjugative plasmid pQBR103 in Pseudomonas fluorescens SBW25 in the plant rhizosphere. This plasmid fitness cost could be ameliorated by compensatory mutations affecting the chromosomal global regulatory system gacA/gacS, which arose rapidly in plant rhizosphere communities and were exclusive to plasmid carriers. These findings expand our understanding of the importance of compensatory evolution in plasmid dynamics beyond simplified lab media. Compensatory mutations contribute to plasmid survival in bacterial populations living within complex microbial communities in their environmental niche. Compensatory mutations reducing the cost of plasmid carriage occur in the plant rhizosphere.
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影响因子：
10.7
作者：
Porse A;Schønning K;Munck C;Sommer MO
通讯作者：
Sommer MO
影响因子：
3.3
作者：
Ross-Gillespie, Adin;Gardner, Andy;Griffin, Ashleigh S.
通讯作者：
Griffin, Ashleigh S.
影响因子：
16.8
作者：
Loftie-Eaton W;Bashford K;Quinn H;Dong K;Millstein J;Hunter S;Thomason MK;Merrikh H;Ponciano JM;Top EM
通讯作者：
Top EM
影响因子：
5.1
作者：
Song, Chunxu;Kidarsa, Teresa A.;Raaijmakers, Jos M.
通讯作者：
Raaijmakers, Jos M.
DOI：
10.1098/rspb.2017.2497
发表时间：
2018-01-10
期刊：
Proceedings. Biological sciences
影响因子：
--
作者：
Stevenson C;Hall JPJ;Brockhurst MA;Harrison E
通讯作者：
Harrison E