Sex peptide of Drosophila melanogaster males is a global regulator of reproductive processes in females
Sex peptide of Drosophila melanogaster males is a global regulator of reproductive processes in females
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DOI：
10.1098/rspb.2012.1634
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发表时间：
2012-11-07
影响因子：
4.7
通讯作者：
Chapman, T.
中科院分区：
文献类型：
--
作者：
Gioti, A.;Wigby, S.;Chapman, T.
精液蛋白（Sfps）改变女性的行为和生理，可以调解性冲突。在黑腹果蝇中，一个单一的Sfp，即性肽（sex peptide，SP），可以引起雌性果蝇交配后的显著反应，包括生殖力、摄食、免疫和性感受性的改变。这些影响可能有利于男性的进化利益，同时在女性中产生成本。我们测试的假设，SP是一个上游的主调节器能够诱导不同的表型，通过有效诱导广泛的转录变化的女性。我们分析mRNA的反应，SP在成年女性腹部（Abd）和头+胸（HT）组织在交配后3和6小时使用微阵列。SP引起了丰富的，微妙的签名的时间和空间控制的mRNA。与卵发育、早期胚胎发生、免疫、营养感测、行为以及出乎意料的光转导相关的基因发生了重大变化。在HT与Abd中，在跨时间点的差异表达方向上存在实质上更多的变化。研究结果支持了SP是女性基因表达的重要调节因子的观点。因此，一种性别中许多基因的表达可能受到另一种性别中表达的调节因子的影响。这可能会影响性冲突的范围内和之间的地点。
Seminal fluid proteins (Sfps) alter female behaviour and physiology and can mediate sexual conflict. In Drosophila melanogaster, a single Sfp, the sex peptide (SP), triggers remarkable post-mating responses in females, including altered fecundity, feeding, immunity and sexual receptivity. These effects can favour the evolutionary interests of males while generating costs in females. We tested the hypothesis that SP is an upstream master-regulator able to induce diverse phenotypes through efficient induction of widespread transcriptional changes in females. We profiled mRNA responses to SP in adult female abdomen (Abd) and head+thorax (HT) tissues using microarrays at 3 and 6 h following mating. SP elicited a rich, subtle signature of temporally and spatially controlled mRNAs. There were significant alterations to genes linked to egg development, early embryogenesis, immunity, nutrient sensing, behaviour and, unexpectedly, phototransduction. There was substantially more variation in the direction of differential expression across time points in the HT versus Abd. The results support the idea that SP is an important regulator of gene expression in females. The expression of many genes in one sex can therefore be under the influence of a regulator expressed in the other. This could influence the extent of sexual conflict both within and between loci.