A multilocus genealogical approach to phylogenetic species recognition in the model eukaryote Neurospora
A multilocus genealogical approach to phylogenetic species recognition in the model eukaryote Neurospora
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DOI：
10.1554/03-073
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发表时间：
2003-12-01
期刊：
影响因子：
3.3
通讯作者：
Taylor, JW
中科院分区：
文献类型：
--
作者：
Dettman, JR;Jacobson, DJ;Taylor, JW
批判性地研究系统发育和生殖相容性标准所认可的物种之间的关系。我们将系统发育物种识别（PSR）应用于生物物种识别（BSR）应用最广泛的真菌，即脉孢菌属。对 147 个个体的四个独立匿名核位点进行了表征和测序，这些个体代表了所有描述的脉孢菌近交物种。我们开发了一种共识树方法，该方法识别出得到大多数基因座一致支持的单系谱系群体，或者得到至少一个基因座的良好支持但不与任何其他基因座相矛盾的单系谱系群体。我们总共识别了8个系统发育物种，其中5个与5个传统生物物种相对应，其中3个是新发现的。系统发育标准不仅在揭示脉孢菌的完整物种多样性方面优于传统的生殖相容性标准，而且在某些物种内检测到了显着的系统发育细分。尽管之前有粗糙猪笼草和中间猪笼草在自然界中杂交的建议，并且事实上本研究中包括了几个假定的杂交个体，但没有观察到支持最近种间基因流或真正杂交体存在的分子证据。将四个基因座的序列数据组合起来，用于阐明 PSR 发现的物种之间的相关性。尽管物种层面的进化枝得到了大力支持，但物种之间的系统发育关系仍然难以解决，这可能是由于差异谱系排序产生的相互冲突的信号所致。
To critically examine the relationship between species recognized by phylogenetic and reproductive compatibility criteria. we applied phylogenetic species recognition (PSR) to the fungus in which biological species recognition (BSR) has been most comprehensively applied, the well-studied genus Neurospora. Four independent anonymous nuclear loci were characterized and sequenced from 147 individuals that were representative of all described outbreeding species of Neurospora. We developed a consensus-tree approach that identified monophyletic genealogical groups that were concordantly supported by the majority of the loci, or were well supported by at least one locus but not contradicted by any other locus. We recognized a total of eight phylogenetic species, five of which corresponded with the five traditional biological species, and three of which were newly discovered. Not only were phylogenetic criteria superior to traditional reproductive compatibility criteria in revealing the full species diversity of Neurospora, but also significant phylogenetic subdivisions were detected within some species. Despite previous suggestions of hybridization between N. crassa and N. intermedia in nature, and the fact that several putative hybrid individuals were included in this study, no molecular evidence in support of recent interspecific gene flow or the existence of true hybrids was observed. The sequence data from the four loci were combined and used to clarify how the species discovered by PSR were related. Although species-level clades were strongly supported, the phylogenetic relationships among species remained difficult to resolve, perhaps due to conflicting signals resulting from differential lineage sorting.