RAPD, RFLP and SSLP analyses of phylogenetic relationships between cultivated and wild species of rice
RAPD, RFLP and SSLP analyses of phylogenetic relationships between cultivated and wild species of rice
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DOI：
10.1266/ggs.76.71
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发表时间：
2001-04-01
影响因子：
1.1
通讯作者：
Ishii, T
中科院分区：
文献类型：
--
作者：
Bautista, NS;Solis, R;Ishii, T
利用随机扩增多态性DNA、限制性片段长度多态性、核SSLP和叶绿体SSLP等分子标记对水稻A基因组种间的亲缘关系进行了分析。共有12个栽培稻品种(4个粳稻、3个爪哇稻和5个籼稻)和1个野生稻品种(12个普通野生稻、2个普通野生稻、1个长退化野生稻、1个子午型野生稻和1个巴氏野生稻)。对它们的带型进行评分和比较，以评估材料之间的相似性。根据平均相似性指数分析了类群内和类群间的遗传分化。除叶绿体SSLP分析外，普通野生稻与普通野生稻的平均相似性均高于普通野生稻，而亚洲野生稻的亚种内变异较大。栽培种与野生种的比较表明，唾液野生稻与普通野生稻有较近的亲缘关系，而斑潜蝇与巴氏野生稻有较近的亲缘关系。这表明两个栽培种，即普通野生稻和非洲野生野生稻，分别起源于普通野生稻和巴氏野生稻。驯化结果表明，日本野生野生稻与中国产的普通野生稻有很强的相似性，而亚洲热带地区的普通野生稻与普通野生野生稻有很强的相似性。此外，还构建了用于RAPD、RFLP以及核和叶绿体SSLP分析的树状图，以揭示A基因组物种之间的总体遗传关系。在所有的分析中，唾液和角果野生稻分别与普通野生稻和巴氏野生稻组成了类群。然而，它们与其他野生物种的聚集方式并不相同。随机扩增多态DNA和限制性片段长度多态性分析的结果表明，普通野生稻与唾液野生稻和非洲野生稻的亲缘关系较近，而长粒野生稻和子午线野生稻与其他A基因组物种有较高的区分度。另一方面，通过核或叶绿体SSLP分析没有获得明显的种间关系。
RAPD, RFLP, nuclear SSLP and chloroplast SSLP analyses were carried out to clarify the phylogenetic relationships among A-genome species of rice. In total, 12 cultivars of Oryza sativa (4 Japonica, 3 Javanica and 5 Indica), one cultivar of O. glaberrima, and 17 wild accessions (12 O. rufipogon, 2 O. glumaepatula, 1 O. longistaminata, 1 O. meridionalis and 1 O. barthii) were used. Their banding patterns were scored and compared to evaluate the similarity between accessions. Genetic differentiation within and between taxa was examined based on the average similarity indices. Except for chloroplast SSLP analysis, the average similarities were higher within O. saliva than within O. rufipogon, and O. saliva Indica had greater intrasubspecific variation than Japonica and Javanica. Comparisons between cultivated and wild species showed that O. saliva was closely related to O. rufipogon, while O. glaberrima was closely related to O. barthii. This indicated that two cultivated species, O. saliva and O. glaberrimia, originated from O. rufipogon and O. barthii, respectively. Domestication of O. saliva seemed to be diphyletic, since strong similarity was observed between O. saliva Japonica-Javanica and O. rufipogon from China and between (3. saliva Indica and O. rufipogon from tropical Asia. In addition, dendrograms for RAPD, RFLP, and nuclear and chloroplast SSLP analyses were constructed to reveal the overall genetic relationships among A-genome species. In all analyses, O. saliva and O. glaberrima formed groups with O. rufipogon and O. barthii, respectively. However, their manners of clustering with other wild species were not the same. The results of RAPD and RFLP analyses indicate that O. glumaepatula was relatively close to the groups of O. saliva and O. glaberrima whereas O. longistaminata and O. meridionalis were highly differentiated from other A-genome species. On the other hand, clear interspecific relationships were not obtained by nuclear or chloroplast SSLP analyses.