Expression of vaspin in the joint and the levels in the serum and synovial fluid of patients with osteoarthritis
Expression of vaspin in the joint and the levels in the serum and synovial fluid of patients with osteoarthritis
复制标题
骨关节炎患者关节中vaspin的表达及血清和滑液中的含量
DOI：
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
吴立东
中科院分区：
文献类型：
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作者：
包家鹏;蒋利锋;陈炜平;胡鹏飞;吴立东
本研究的目的是确定vaspin在关节中的表达，并研究骨关节炎（OA）患者配对血清和滑液（SF）以及健康对照血清之间的分布。通过定量实时聚合酶链反应（qPCR）测定OA关节组织中vaspin的基因表达。采用免疫组织化学方法检测OA患者全膝关节置换术后软骨、滑膜和骨赘中vaspin蛋白的表达。采用酶联免疫吸附法（ELISA）检测26例OA患者和23例健康对照者血清和SF中vaspin的水平。OA患者关节组织包括软骨、滑膜、半月板、髌下脂肪垫和骨赘均表达vaspin mRNA，OA软骨、滑膜和骨赘中均有vaspin蛋白表达。此外，与健康对照相比，OA患者的血清vaspin降低，并且OA患者的血清vaspin水平超过配对SF中的水平。血清或SF vaspin与年龄、性别或体重指数（BMI）无关。这些结果表明，vaspin可能参与OA的病理生理学，并可能在OA过程中对关节产生局部影响。
The aim of this study was to determine the expression of vaspin in the joint and investigate the distribution between paired serum and synovial fluid (SF) in osteoarthritis (OA) patients, and serum in healthy controls. The gene expression of vaspin was measured by quantitative real-time polymerase chain reaction (qPCR) in the OA joint tissues. The vaspin protein expression in the cartilage, synovium and osteophyte from OA patients who required total knee replacement (TKR) were detected by immunohistochemistry (IHC). Levels of vaspin in serum and SF were analyzed by enzyme-linked immunosorbent assay (ELISA), including 26 OA patients and 23 healthy controls. All the joint tissues including cartilage, synovium, meniscus, infrapatellar fat pad and osteophyte from OA patients expressed vaspin messenger RNA (mRNA), and the expression of vaspin protein was observed in OA cartilage, synovium and osteophyte. Furthermore, serum vaspin was reduced in OA patients compared to healthy controls, and serum vaspin levels from OA patients exceed those in the paired SF. Serum or SF vaspin were not related to age, gender, or body mass index (BMI). These results suggest that vaspin may be involved in the pathophysiology of OA and may have local effects in the joint during the process of OA.