The PCP pathway regulates Baz planar distribution in epithelial cells
The PCP pathway regulates Baz planar distribution in epithelial cells
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DOI：
10.1038/srep33420
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发表时间：
2016-09-14
影响因子：
4.6
通讯作者：
Le Bivic, Andre
中科院分区：
文献类型：
--
作者：
Aigouy, Benoit;Le Bivic, Andre
顶基两极蛋白沿上皮细胞Z轴的定位已为人所熟知，但其在上皮平面上的分布特征尚不清楚。在这里，我们系统地描述了果蝇眼上皮中顶-基极性蛋白的平面定位。我们发现粘着连接蛋白ShotGun和Armadillo以及基底-侧向复合体是双边的，即存在于细胞界面的两侧。相反，我们报告了其他关键的黏附连接蛋白，Bazooka和肌球蛋白调节轻链(意大利面南瓜)是单边的，即存在于细胞界面的一侧。此外，我们证明了平面细胞极性(PCP)而不是顶端决定因素Crumbs和PAR-6控制了锥体细胞的Bazooka单边性。总之，我们的工作揭示了一种意想不到的组织和组合，即尖基、细胞骨架和平面极性蛋白质，这种蛋白质在细胞-细胞界面的两侧是不同的，对于同一细胞的不同接触是独特的。
The localisation of apico-basal polarity proteins along the Z-axis of epithelial cells is well understood while their distribution in the plane of the epithelium is poorly characterised. Here we provide a systematic description of the planar localisation of apico-basal polarity proteins in the Drosophila ommatidial epithelium. We show that the adherens junction proteins Shotgun and Armadillo, as well as the baso-lateral complexes, are bilateral, i.e. present on both sides of cell interfaces. In contrast, we report that other key adherens junction proteins, Bazooka and the myosin regulatory light chain (Spaghetti squash) are unilateral, i.e. present on one side of cell interfaces. Furthermore, we demonstrate that planar cell polarity (PCP) and not the apical determinants Crumbs and Par-6 control Bazooka unilaterality in cone cells. Altogether, our work unravels an unexpected organisation and combination of apico-basal, cytoskeletal and planar polarity proteins that is different on either side of cell-cell interfaces and unique for the different contacts of the same cell.