THE NEUTROPHIL-ACTIVATING PROTEIN (NAP-1) IS ALSO CHEMOTACTIC FOR LYMPHOCYTES-T
THE NEUTROPHIL-ACTIVATING PROTEIN (NAP-1) IS ALSO CHEMOTACTIC FOR LYMPHOCYTES-T
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DOI：
10.1126/science.2648569
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发表时间：
1989-03-17
期刊：
影响因子：
56.9
通讯作者：
MATSUSHIMA, K
中科院分区：
文献类型：
--
作者：
LARSEN, CG;ANDERSON, AO;MATSUSHIMA, K
用植物血凝素刺激人血单个核白细胞的条件培养液，经一系列层析纯化T淋巴细胞趋化因子（TCF）。纯化的TCF的氨基端氨基酸序列显示与嗜中性粒细胞活化蛋白（NAP-1）的同一性。TCF和重组NAP-1（rNAP-1）在体外对中性粒细胞和T淋巴细胞具有趋化性，支持TCF与NAP-1的同一性。将rNAP-1注射到Fisher大鼠淋巴结的淋巴引流区中，仅引起高内皮微静脉中淋巴细胞的加速迁移。皮内注射rNAP-1引起中性粒细胞和淋巴细胞的剂量依赖性积累。
T lymphocyte chemotactic factor (TCF) was purified to homogeneity from the conditioned media of phytohemagglutinin-stimulated human blood mononuclear leukocytes by a sequence of chromatography procedures. The amino-terminal amino acid sequence of the purified TCF showed identity with neutrophil-activating protein (NAP-1). Both TCF and recombinant NAP-1 (rNAP-1) were chemotactic for neutrophils and T lymphocytes in vitro supporting the identity of TCF with NAP-1. Injection of rNAP-1 into lymphatic drainage areas of lymph nodes in Fisher rats caused accelerated emigration of only lymphocytes in high endothelial venules. Intradermal injection of rNAP-1 caused dose-dependent accumulation of neutrophils and lymphocytes.