An effective zinc phthalocyanine derivative for photodynamic antimicrobial chemotherapy
An effective zinc phthalocyanine derivative for photodynamic antimicrobial chemotherapy
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用于光动力抗菌化疗的有效锌酞菁衍生物
DOI：
10.1016/j.jlumin.2013.10.067
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发表时间：
2014-08-01
影响因子：
3.6
通讯作者：
Huang, Mingdong
中科院分区：
文献类型：
--
作者：
Chen, Zhuo;Zhou, Shanyong;Huang, Mingdong
细菌感染是临床常见问题。抗生素耐药细菌的出现给全世界的医疗实践带来了严峻的挑战。光动力抗菌化疗（PACT）利用特定波长的激光将人体组织中的氧分子激活为活性氧作为抗菌剂。激光对氧气的激活是通过光敏剂介导的。强效光敏剂的两个关键特性是其对红外区域（630-700 nm）的光的吸收，从而促进组织渗透深度，以及选择性地积聚在细菌而不是人体组织上。我们在此报告了一种锌酞菁衍生物，五赖氨酸β-羰基酞菁锌（ZnPc-(Lys)(5)）及其体外和动物感染模型中的抗菌作用。该光敏剂在670 nm处具有很强的杀菌能力。从化学角度看，它是一种在生理pH下带正电荷的水溶性阳离子光敏剂，可以特异性地靶向表面通常带负电荷的细菌。与阴离子ZnPc对应物相比，ZnPc-(Lys)(5)对细菌表现出更高的光毒性。 ZnPc-(Lys)(5) 在实验感染动物模型中的 PACT 研究表明，与对照相比，ZnPc-(Lys)(5) 具有显着的细菌抑制作用，并且 ZnPc-(Lys)(5) 对细菌具有高选择性。这些发现表明 ZnPc-(Lys)(5) 是一种有前途的用于治疗传染病的抗菌光敏剂。 (C) 2013 Elsevier B.V. 保留所有权利。
Bacterial infection is a common clinical problem. The emergence of antibiotic resistant bacteria posts a severe challenge to medical practice worldwide. Photodynamic antimicrobial chemotherapy (PACT) uses laser light at specific wavelength to activate oxygen molecule in the human tissue into reactive oxygen species as antimicrobial agent. This activation of oxygen by laser light is mediated through a photosensitizer. Two key properties for potent photosensitizer are its absorbance of light in the infrared region (630-700 nm), which promotes tissue penetration depth, and the selective accumulation on bacteria instead of human tissue. We herein report a zinc phthalocyanine derivative, pentalysine beta-carbonylphthalocyanine zinc (ZnPc-(Lys)(5)) and its antimicrobial effects in vitro and in an animal infection model. This photosensitizer has strong capability to kill bacteria at 670 nm. Chemically, it is a water-soluble and cationic photosensitizer carrying positive charge under physiological pH, and can specifically target to bacteria which usually bears negative charges on its surface. Compared with anionic ZnPc counterparts, ZnPc-(Lys)(5) shows a higher phototoxicity toward bacteria. PACT studies of ZnPc-(Lys)(5) in experimental infection animal model showed a significant bacteria inhibition compared to controls, and high selectivity of ZnPc-(Lys)(5) toward bacteria. These findings suggest ZnPc-(Lys)(5) is a promising antimicrobial photosensitizer for the treatment of infectious diseases. (C) 2013 Elsevier B.V. All rights reserved.