Comparative application of antibody and gene array for expression profiling in human squamous cell lung carcinoma
Comparative application of antibody and gene array for expression profiling in human squamous cell lung carcinoma
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DOI：
10.1016/j.lungcan.2005.02.006
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发表时间：
2005-08-01
期刊：
影响因子：
5.3
通讯作者：
Simm, A
中科院分区：
文献类型：
--
作者：
Bartling, B;Hofmann, HS;Simm, A
基因微阵列技术的表达受益已被开发用于预测恶性组织，但还没有应用抗体阵列来识别恶性肿瘤相关蛋白的经验。由于改变的蛋白质模式也可能更好地解释生物过程，因此我们将 12 名鳞状细胞肺癌患者的肿瘤样本和个体肺组织对照应用于带有 378 种不同单克隆抗体的抗体阵列。阵列分析确定了肺肿瘤中丰度较高的 20 种蛋白质和丰度较低的 9 种蛋白质。与基因微阵列数据的比较显示，31% 的差异调节蛋白与鳞状细胞肺癌中 mRNA 表达的改变相关，包括上调蛋白的 PEX1、MKK7 和 HDAC3。通过免疫印迹分析对组蛋白脱乙酰酶 (HDAC) 3 进行了详细研究，结果表明 HDAC3 在 92% 的肿瘤中确实升高（n=22/24；P
Expression profiting by gene microarray techniques have been developed to predict malignant tissue but there are no experiences with the application of antibody arrays to identify malignancy-related proteins. Because altered protein patterns might also better interpret biological processes, we applied tumour samples from 12 patients with squamous cell lung carcinoma and individual lung tissue controls to antibody arrays spotted with 378 distinct monoclonal antibodies. Array analysis defined 20 proteins with higher and nine with lower abundance in lung tumours. Comparison with gene microarray data revealed that 31% of the differentially regulated proteins correlate with altered mRNA expression in squamous cell lung carcinomas, including PEX1, MKK7 and HDAC3 for up-regulated proteins. The histone deacetylase (HDAC) 3 was investigated in detail by immunoblot analysis showing that HDAC3 is indeed elevated in 92% of tumours (n=22/24; P