TEMPERATURE EFFECTS ON GROWTH, DOMOIC ACID PRODUCTION, AND MORPHOLOGY OF THE DIATOM Nitzschia pungens f. multiseries
TEMPERATURE EFFECTS ON GROWTH, DOMOIC ACID PRODUCTION, AND MORPHOLOGY OF THE DIATOM Nitzschia pungens f. multiseries
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温度对硅藻 Nitzschia pungens f 生长、软骨藻酸产生和形态的影响。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
J. Smith
中科院分区：
文献类型：
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作者：
N. Lewis;S. Bates;J. Mclachlan;J. Smith
产藻酸（DA）的pennate硅藻Nitzschia pungens f. multiseries（克隆POM，来自加拿大Nova Scotia）在光子通量密度（PFD）为80和180 IlE m- 2 S-1和5°，10°，15°，20°和25°C的条件下生长。生长速率（Il）从5℃时的0.25 d- 1增加到20℃时的最大值0.65 d- 1；在25°C时为0.60 d-'。两种PFD在任何温度下培养的Il均无显著差异。在5°~ 15°C和10°~ 20°C范围内，Il的0.10分别为1.76和2.06。固定相细胞浓度在5°至15°C时最高，在20°至25°C时下降。DA产率从5℃时的0.01 pg DA cell ' d- 1提高到25℃时的0.51 pg DA cell ' d- 1；在5°~ 15°C和15°~ 25°C范围内，0.10分别为19.6和2.11。扫描电镜显示，细胞在5°和15°C时，肋间有3 - 4排孔。相反，在25°C时，细胞有2 - 3行多孔体。在较低温度下较高的细胞浓度可能会赋予刺毛霉竞争优势，尽管在加拿大东部秋冬自然栖息地的低温环境中，刺毛霉的生长或DA的产生似乎没有独特的生理适应。
The domoic acid (DA) producing pennate diatom Nitzschia pungens f. multiseries (clone POM, from Nova Scotia, Canada) was grown at photon flux densities (PFD) of 80 and 180 IlE m- 2 S-1 and 5°,10°,15°,20° and 25°C. The growth rate (Il) increased from 0.25 d" at 5°C to a maximum of 0.65 d- 1 at 20°C; it was 0.60 d-' at 25°C. There was no significant difference in Il between cultures grown at the two PFD at any temperature. The 0 10 for Il was 1.76 and 2.06 between 5° - 15°C and 10° - 20°C, respectively. The highest stationary phase cell concentrations occurred at 5° to 15°C and decreased at 20° to 25°C. The DA production rate increased from 0.01 pg DA cell" d- 1 at 5°C to 0.51 pg DA ceW' d-' at 25°C; the 0 10 was 19.6 and 2.11 between 5° - 15°C and 15° 25°C, respectively. Scanning electron microscopy showed that cells at 5° and 15°C had 3 - 4 rows of poroids between the costae. In contrast, cells at 25°C had 2 - 3 rows of poroids. The higher cell concentrations at lower temperatures may confer a competitive advantage to N. pungens, although there appears to be no unique physiological adaptation related to growth or DA production at the low temperatures characteristic of its natural habitat in autumn and winter in eastern Canada.