ACTIVE RECOMBINANT C3A OF HUMAN ANAPHYLATOXIN PRODUCED IN ESCHERICHIA-COLI
ACTIVE RECOMBINANT C3A OF HUMAN ANAPHYLATOXIN PRODUCED IN ESCHERICHIA-COLI
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DOI：
10.1016/0006-291x(91)91683-4
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发表时间：
1991-03-29
影响因子：
3.1
通讯作者：
TACHIBANA, T
中科院分区：
文献类型：
--
作者：
FUKUOKA, Y;YASUI, A;TACHIBANA, T
编码77个氨基酸的完整人C3 a的DNA序列被分成三部分，分别合成并构建用于在大肠杆菌中表达。利用T7聚合酶的表达系统实现了重组C3a的高效表达。纯化的重组C3a具有与人血清中纯化的C3a相同的豚鼠回肠收缩和血小板聚集活性。
DNA sequence coding for the complete human C3a with 77 amino acids was divided into three portions, synthesized separately and constructed for expression inEscherichia coli. High expression of the recombinant C3a was achieved by an expression system using T7 polymerase. Purified recombinant C3a showed the same activities of ileum contraction and platelet aggregation of guinea pig as C3a purified from human serum.