Acylglycerol kinase is over-expressed in early-stage cervical squamous cell cancer and predicts poor prognosis
Acylglycerol kinase is over-expressed in early-stage cervical squamous cell cancer and predicts poor prognosis
复制标题
酰基甘油激酶在早期宫颈鳞状细胞癌中过度表达，预示预后不良
DOI：
10.1007/s13277-015-4498-4
复制
发表时间：
2016-05-01
期刊：
影响因子：
--
通讯作者：
Yu, Yan-Hong
中科院分区：
文献类型：
--
作者：
Sun, Fei;Xiong, Ying;Yu, Yan-Hong
酰基甘油激酶（Acylglycerol kinase，AGK）已被证明与癌症进展和患者的不利临床结果有关。本研究旨在探讨AGK在早期宫颈鳞状细胞癌（CSCC）中的表达及其临床意义。采用蛋白质印迹法和实时PCR（RT-PCR）分析了六种宫颈癌细胞系和四对早期CSCC标本和正常宫颈组织（NCT）中AGK的蛋白和信使RNA（mRNA）表达。采用免疫组化方法检测140例早期CSCC和30例NCT石蜡包埋标本中AGK蛋白的表达。统计分析AGK表达的临床病理意义。AGK蛋白和mRNA在宫颈癌细胞系和癌组织中的表达均显著上调。免疫组化分析显示，AGK在140例早期CSCC标本中有93例（66.4%）高表达，但在NCT中无表达。AGK在早期CSCC中的表达与肿瘤分期（P< 0.001）、肿瘤大小（P< 0.001）和肿瘤类型（P< 0.001）有关。AGK高表达的早期CSCC患者与AGK低表达的患者相比，无进展生存期（PFS）和总生存期（OS）较短。单因素和多因素分析表明，AGK表达水平是早期CSCC患者生存的独立预后因素。我们发现AGK在宫颈癌细胞系和临床组织中过表达，并且AGK的过表达与早期CSCC患者的不良生存结局相关。AGK可作为早期CSCC独立的预后指标。
Acylglycerol kinase (AGK) had been shown to contribute to cancer progression and unfavorable clinical outcomes of patients. Our study aimed to investigate the expression pattern and clinical significance of AGK in patients with early-stage cervical squamous cell cancer (CSCC). The protein and messenger RNA (mRNA) expression of AGK was analyzed in six cervical cancer cell lines and four paired early-stage CSCC specimens and normal cervical tissues (NCT), using Western blotting and real-time PCR (RT-PCR). And we investigated the AGK protein expression in paraffin-embedded specimens from 140 patients with early-stage CSCC and 30 cases of NCT by immunohistochemistry (IHC). Statistical analyses were performed to evaluate the clinicopathological significance of AGK expression. The expressions of AGK protein and mRNA were significantly up-regulated in cervical cancer cell lines and cancer tissues. IHC analyses revealed that AGK was highly expressed in 93 (66.4 %) of 140 early-stage CSCC specimens, but in none of the NCT. Moreover, AGK expression in early-stage CSCC was significantly correlated with tumor stage (P< 0.001), tumor size (P< 0.001), and tumor type (P< 0.001). Early-stage CSCC patients with high AGK expression level had shorter progress-free survival (PFS) and overall survival (OS) time compared with patients with low AGK expression levels. Univariate and multivariate analyses identified AGK expression level as an independent prognostic factor for survival of early-stage CSCC patients. We showed that AGK was over-expressed in cervical cancer cell lines and clinical tissues, and over-expression of AGK was associated with poor survival outcomes of early-stage CSCC patients. AGK can be used as an independent prognostic marker for early-stage CSCC.