Oxidative Stress in Rat Testis and Epididymis Under Intermittent Hypobaric Hypoxia: Protective Role of Ascorbate Supplementation
Oxidative Stress in Rat Testis and Epididymis Under Intermittent Hypobaric Hypoxia: Protective Role of Ascorbate Supplementation
复制标题
DOI：
10.2164/jandrol.108.007054
复制
发表时间：
2010-05-01
影响因子：
--
通讯作者：
Reyes, Juan G.
中科院分区：
文献类型：
--
作者：
Gonzalo Farias, Jorge;Puebla, Mariela;Reyes, Juan G.
低压缺氧（Hypobaric hypoxia，HH）是登山者、矿工、天文台、乡村卫生、边防巡逻和乡村教育工作者所面临的一种高海拔环境条件，它危及人体的正常生理功能。本研究旨在评价间歇性HH（IHH;相当于平均海拔4600 m）对大鼠睾丸和附睾氧化应激的影响以及膳食抗坏血酸的保护作用。将10周龄雄性Wistar大鼠分为6组：1）常压（Nx），2）Nx +生理溶液（Nx + PS），3）Nx +抗坏血酸（Nx + AA），4）IHH，5）IHH + PS，或6）IHH + AA。将接受IHH的动物暴露96小时，然后在常压条件下暴露96小时，共32天。对照组（2和5）注射一定剂量的PS，治疗组（3和6）注射一定剂量的AA（10 mg × kg（-1）体重），间隔96小时。在方案开始后第32天处死大鼠。收集睾丸和附睾，测定谷胱甘肽还原酶的活性和表达以及脂质过氧化物的形成水平。还对每只动物进行了附睾精子计数。本研究的结果显示，IHH诱导脂质过氧化，减少谷胱甘肽还原酶活性在睾丸和附睾，并在附睾精子计数显着下降。AA治疗阻止了这些变化。总之，AA能够降低IHH下睾丸和附睾的氧化应激。这种保护由AA的IH诱导的脂质过氧化反应可以部分地解释由谷胱甘肽还原酶活性在这些器官中的保存。
Hypobaric hypoxia (HH), an environmental condition of high altitude encountered by mountaineers, miners, and observatory, rural health, border patrol, and rural education workers, jeopardizes normal physiologic functions in humans. The present study was conducted to evaluate the effects of intermittent HH (IHH; equivalent to 4600 m above mean sea level) on oxidative stress and the protective role of dietary ascorbic acid on rat testis and epididymis. Ten-week-old male Wistar rats were assigned to 1 of 6 groups: 1) normobaric (Nx), 2) Nx + physiologic solution (Nx + PS), 3) Nx + ascorbic acid (Nx + AA), 4) IHH, 5) IHH + PS, or 6) IHH + AA. Animals subjected to IHH were exposed for 96 hours followed by normobaric conditions for 96 hours for a total of 32 days. The control groups (2 and 5) were injected with doses of PS, and the treated groups (3 and 6) were injected with doses of AA (10 mg x kg(-1) body weight) at an interval of 96 hours. Rats were sacrificed on day 32 after initiation of the protocol. The testis and epididymis were collected to determine the activity and expression of glutathione reductase and the levels of lipid peroxide formation. An epididymal sperm count was also performed in each animal. The results of this study revealed that IHH induced lipid peroxidation, a reduction in glutathione reductase activity in testis and epididymis, and a significant decrease in epididymal sperm count. Treatment with AA prevented these changes. In conclusion, AA was capable of decreasing oxidative stress in testis and epididymis under IHH. This protection by AA of the IHH-induced lipid peroxidation can be explained in part by the preservation of glutathione reductase activity in these organs.