Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits
Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits
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DOI：
10.1042/bst0320899
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发表时间：
2004-12-01
影响因子：
3.9
通讯作者：
Workman, JL
中科院分区：
文献类型：
--
作者：
Lee, KK;Prochasson, P;Workman, JL
表观遗传学是指在不影响基因型的情况下改变表型。表观遗传学的潜在分子机制是共价组蛋白修饰和核小体重组引起染色质结构的改变。在酵母中，研究得最充分的两个执行这些表观遗传变化的大分子复合物是atp依赖性Swi/Snf染色质重塑复合物和SAGA组蛋白乙酰转移酶复合物。为了充分了解这些大蛋白复合物在细胞中发挥作用的机制，鉴定这些复合物的所有亚基是至关重要的。为了鉴定与SAGA和Swi/Snf相关的新亚基，我们使用串联亲和纯化，然后使用多维蛋白鉴定技术分析亚基组成。我们的分析发现了两个新的蛋白，一个与SAGA相关，YPL047W (Sgf 11)，另一个与Swi/Snf相关，Rtt102。
Epigenetics is the alteration of phenotype without affecting the genotype. An underlying molecular mechanism of epigenetics is the changes of chromatin structure by covalent histone modifications and nucleosome reorganization. In the yeast, Soccharomyces cerevisiae, two of the most well-studied macromolecular complexes that perform these epigenetic changes are the ATP-dependent Swi/Snf chromatin-remodelling complex and the SAGA histone acetyltransferase complex. To understand fully the mechanism by which these large protein complexes perform their functions in the cell, it is crucial that all the subunits of these complexes are identified. In an attempt to identify new subunits associated with SAGA and Swi/Snf, we used tandem affinity purification, followed by a multidimensional protein identification technology to analyse the subunit composition. Our analysis identified two novel proteins, one associated with SAGA, YPL047W (Sgf 11), and another associated with Swi/Snf, Rtt102.