A class IIa peptide from Enterococcus mundtii inhibits bacteria associated with otitis media
A class IIa peptide from Enterococcus mundtii inhibits bacteria associated with otitis media
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DOI：
10.1016/j.ijantimicag.2007.10.010
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发表时间：
2008-03-01
影响因子：
10.8
通讯作者：
Dicks, L. M. T.
中科院分区：
文献类型：
--
作者：
Knoetze, H.;Todorov, S. D.;Dicks, L. M. T.
由蒙氏肠球菌ST 4SA产生的肽ST 4SA抑制鲍曼不动杆菌、粪肠球菌、屎肠球菌、金黄色葡萄球菌、肺炎链球菌和从诊断患有中耳感染的患者分离的革兰氏阳性菌的生长。该肽对S.肺炎假单胞菌40株、铜绿假单胞菌25株、大肠杆菌40株。屎藓低浓度的ST 4SA（51 200任意单位（Au）/mL）引起靶细胞的DNA和酶泄漏，而1 638 400 Au/mL引起细胞裂解。在以人血为基质的固体培养基上检测时，未观察到抗微生物活性降低。肽ST 4SA显示出与四环素（30 μ g）相似的活性水平，但当在体外测试时，与鼻喷雾剂、氨基糖苷类、头孢菌素类、氟喹诺酮类、林可酰胺类、大环内酯类、硝基咪唑类、青霉素、喹诺酮类、磺胺类、氯霉素、呋喃唑酮、夫西地酸、利福平、甲氧苄啶、甲氧苄啶/磺胺甲恶唑和万古霉素相比活性高得多。肽ST 4SA消散质子动力，并且可用于治疗多药耐药菌株，其中抗生素通过依赖于膜质子梯度的外排泵从细胞中排除。(C)2007年爱思唯尔B。和国际化疗学会。All rights reserved.
Peptide ST4SA, produced by Enterococcus mundtii ST4SA, inhibits the growth of Acinetobacter baumannii, Enterococcus faecalis, Enterococcus faecium, Staphylococcus aureus, Streptococcus pneumoniae and Gram-positive bacteria isolated from patients diagnosed with middle ear infections. The peptide adsorbed at a level of 94% to S. pneumoniae 40, Pseudomonas aeruginosa 25 and E. faecium HKLHS. Low concentrations of peptide ST4SA ( 51 200 arbitrary units (AU)/mL) caused DNA and enzyme leakage from target cells, whilst 1 638 400AU/mL caused cell lysis. No decrease in antimicrobial activity was observed when tested on solid medium with human blood as base. Peptide ST4SA revealed a similar level of activity compared with tetracycline (30 mu g), but much higher activity compared with nasal sprays, aminoglycosides, cephalosporins, fluoroquinolones, lincosamides, macrolides, nitroimidazole, penicillin, quinolones, sulphonamides, chloramphenicol, furazolidone, fusidic acid, rifampicin, trimethoprim, trimethoprim/sulfamethoxazole and vancomycin when tested in vitro. Peptide ST4SA dissipates the proton-motive force and may be used in the treatment of multidrug-resistant strains where antibiotics are excluded from cells by efflux pumps dependent on the membrane proton gradient. (C) 2007 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.