Non-primate hepacivirus transmission and prevalence: Novel findings of virus circulation in horses and dogs in Morocco
Non-primate hepacivirus transmission and prevalence: Novel findings of virus circulation in horses and dogs in Morocco
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DOI：
10.1016/j.meegid.2021.104975
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发表时间：
2021-06-27
影响因子：
3.2
通讯作者：
Ezzikouri, Sayeh
中科院分区：
文献类型：
--
作者：
Abbadi, Islam;Lkhider, Mustapha;Ezzikouri, Sayeh
非灵长类肝炎病毒 (NPHV) 是丙型肝炎病毒的同源物，已从狗和马中分离出来。有关 NPHV 患病率和分布的数据并不完整，尤其缺乏来自非洲大陆的报告。本研究是对北非马和狗中 NPHV 流行情况的首次调查。从摩洛哥不同地点的 172 匹马和 36 只狗采集血液，使用针对 5'UTR 和 NS3 区域的巢式 PCR 筛选 NPHV RNA，并使用 Gaussia 荧光素酶免疫沉淀系统分析抗 NPHV NS3 抗体，以确定血清阳性率。从阳性血清样本中分离出的 NS3 区域的八个序列被用于系统发育分析。马和狗的NPHV RNA阳性率分别为10.5%和5.5%，血清阳性率分别为65.7%和8.33%。幼年马似乎更容易受到感染，NHPV RNA 阳性率为 23.5%。母马的血清阳性率比种马更广泛（77.14% vs. 46.27%，p < 0.0001）。从系统发育角度来看，从马和狗中分离出来的 NPHV NS3 基因聚集在一起。我们检测到的 NPHV 毒株与摩洛哥境内的地理位置没有相关性。总之，摩洛哥马表现出许多先前和/或当前 NPHV 感染的证据，其中年轻和女性是潜在的危险因素。有趣的是，NPHV 在狗和马中传播，这表明它已经跨越了物种障碍，并且马和狗是丙型肝炎病毒祖先传播到人群中的潜在载体。
Non-primate hepacivirus (NPHV) is a homolog of hepatitis C virus and has been isolated from dogs and horses. Data on NPHV prevalence and distribution are not complete, and there is a particular lack of reports from the African continent. The present study represents the first investigation of NPHV prevalence in horses and dogs in North Africa. Blood was collected from 172 horses and 36 dogs at different locations in Morocco, and screened for NPHV RNA using nested PCR targeting 5'UTR and NS3 regions and analyzed for anti-NPHV NS3 antibody using a Gaussia luciferase immunoprecipitation system-to determine seroprevalence. Eight sequences of the NS3 region isolated from positive serum samples were targeted for phylogenetic analysis. Horses and dogs showed respective NPHV RNA positivity rates of 10.5% and 5.5%, and seroprevalences of 65.7% and 8.33%. Juvenile horses appeared more susceptible to infection, with a 23.5% NHPV RNA positivity rate. Seropositivity was more extensive in mares than stallions (77.14% vs. 46.27%, p < 0.0001). Phylogenetically, that NPHV NS3 genes isolated from horses and dog are clustered together. The NPHV strains we detected showed no correlation with geographic location within Morocco. In conclusion, Moroccan horses showed much evidence of previous and/or current NPHV infection, with young age and female sex as noted potential risk factors. Interestingly, NPHV is circulating in dogs as well as horses, suggesting that it has crossed species barriers and that horses and dogs are potential vectors by which an ancestor to hepatitis C virus was transmitted into human populations.