Effects of taxol and Colcemid on myofibrillogenesis.
Effects of taxol and Colcemid on myofibrillogenesis.
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紫杉醇和秋水酰胺对肌原纤维形成的影响。
DOI：
10.1073/pnas.79.21.6556
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发表时间：
1982
影响因子：
11.1
通讯作者：
Holtzer,H
中科院分区：
文献类型：
--
作者：
Toyama,Y;Forry-Schaudies,S;Hoffman,B;Holtzer,H
为了确定肌原纤维形成过程中细丝、z带、微管、中间丝（if）、t管和肌浆网（SR）之间的关系，用12-十四烷醇13-乙酸酯（TPA）选择性地耗尽肌原纤维，然后在(i)正常培养基、（ii）紫杉醇和（iii） Colcemid中再生肌原纤维。在正常培养基中组装的肌原纤维形成典型的A-、I-、Z-、M-和h -带以及相关的if、t -管和sr。在紫杉醇中组装的肌原纤维形成与长微管交错排列的粗细丝的“A带”和仅由微管组成的“I带”。这些前所未见的肌瘤缺乏细丝、z带以及相关的IFs和SR。观察到“孤立a带”，完全由横向排列的双极厚丝组成，长度为1.6微米，没有薄丝或微管。肌原纤维在没有微管的情况下形成了所有肌原纤维成分，但这些成分没有达到严格的横向排列。秋碱和紫杉醇诱导形成斑块状的z带，这些z带总是作为细丝的插入位点，而不管邻近的粗丝是否存在。z带可以作为每个肌节的动作组织中心。
To determine the relationship between thin filaments, Z-bands, microtubules, intermediate filaments (IFs), T-tubules, and sarcoplasmic reticulum (SR) during myofibrillogenesis, myotubes were selectively depleted of their myofibrils with 12-tetradecanoylphorbol 13-acetate (TPA) and then were allowed to regenerate in (i) normal medium, (ii) taxol, and (iii) Colcemid. Myofibrils assembled in normal medium formed typical A-, I-, Z-, M-, and H-bands and associated IFs, T-tubules, and SR. Myofibrils assembled in taxol formed "A-bands" of aligned thick filaments interdigitating with long microtubules and "I-bands" consisting only of microtubules. These unprecedented sarcomeres lacked thin filaments, Z-bands, and associated IFs and SR. "Solitary A-bands," consisting exclusively of laterally aligned bipolar thick filaments 1.6 microM in length without either thin filaments or microtubules, were observed. Myofibrils assembled in Colcemid formed all myofibrillar components in the absence of microtubules but these did not achieve rigorous lateral alignment. Colcemid and taxol induced the formation of patchy Z-bands that invariably served as insertion sites for thin filaments, irrespective of the presence or absence of adjacent thick filaments. Z-bands may function as actin-organizing centers for each sarcomere.