Cardiotoxin Induced Injury and Skeletal Muscle Regeneration
Cardiotoxin Induced Injury and Skeletal Muscle Regeneration
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DOI：
10.1007/978-1-4939-3810-0_6
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发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Garry, Daniel J.
中科院分区：
文献类型：
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作者：
Garry, Glynnis A.;Antony, Marie Lue;Garry, Daniel J.
骨骼肌对损伤有巨大的修复和再生能力。这种再生能力在很大程度上是由于肌源性干细胞群体，被称为卫星细胞，居住在成人骨骼肌中。为了破译控制肌源性干细胞静止、激活、分化和自我更新的机制，需要一个可复制的损伤模型。因此，我们利用肌坏死因子心脏毒素的传递来研究肌源性干细胞对损伤的分子机制。在这里，我们描述了我们使用心脏毒素作为一种有效的肌肉坏死剂来研究骨骼肌再生的经验。我们提供了一个详细的方案来检查骨骼肌损伤和再生使用形态分析。
Skeletal muscles have a tremendous capacity for repair and regeneration in response to injury. This capacity for regeneration is largely due to a myogenic stem cell population, termed satellite cells, which are resident in adult skeletal muscles. In order to decipher the mechanisms that govern myogenic stem cell quiescence, activation, differentiation, and self-renewal, a reproducible injury model is required. Therefore, we have utilized the delivery of the myonecrotic agent, cardiotoxin, to examine the molecular mechanisms of myogenic stem cells in response to injury. Here, we describe our experience using cardiotoxin as a potent myonecrotic agent to study skeletal muscle regeneration. We provide a detailed protocol to examine skeletal muscle injury and regeneration using morphological analyses.