Telomeres of the linear chromosomes of Lyme disease spirochaetes: nucleotide sequence and possible exchange with linear plasmid telomeres
Telomeres of the linear chromosomes of Lyme disease spirochaetes: nucleotide sequence and possible exchange with linear plasmid telomeres
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DOI：
10.1046/j.1365-2958.1997.6051963.x
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发表时间：
1997-11-01
影响因子：
3.6
通讯作者：
Huang, WM
中科院分区：
文献类型：
--
作者：
Casjens, S;Murphy, M;Huang, WM
螺旋体属疏螺旋体的细菌具有大小约950kbp的线性染色体。我们在这里报告，这些线性染色体在其末端共价闭合的发夹结构是相似的，但不相同的线性质粒所携带的这些生物体的报告。染色体端粒区的核苷酸序列分析表明，独特的，显然是功能基因位于几百个碱基对的每个端粒，并有一个不完美的26 bp的反向重复在两个端粒。此外，我们的特点是一个主要的染色体长度多态性的权利端粒区域内的各种疏螺旋体菌株，并显示，序列相似的战斗端粒附近的线性质粒上经常发现在B。严格意义上的burgdorferi与自然界隔离。在B上没有发现与染色体其他区域相似的序列，包括靠近左侧端粒的序列。伯氏质粒。这些观察结果表明，线性质粒和线性染色体右端之间存在遗传信息的历史交换。
Bacteria of the spirochaete genus Borrelia have linear chromosomes about 950 kbp in size. We report here that these linear chromosomes have covalently closed hairpin structures at their termini that are similar but not identical to those reported for linear plasmids carried by these organisms. Nucleotide sequence analysis of the chromosomal telomeric regions indicates that unique, apparently functional genes lie within a few hundred bp of each of the telomeres, and that there is an imperfect 26 bp inverted repeat at the two telomeres. In addition, we characterize a major chromosomal length polymorphism within the right telomeric regions of various Borrelia isolates, and show that sequences similar to those near the Fight telomere are often found on linear plasmids in B. burgdorferi (sensu stricto) isolates from nature. Sequences similar to a number of other regions of the chromosome, including those near the left telomere, were not found on B. burgdorferi plasmids. These observations suggest that there has been historical exchange of genetic information between the linear plasmids and the right end of the linear chromosome.