A Quantitative Proteomic Analysis of Brassinosteroid-induced Protein Phosphorylation in Rice (Oryza sativa L.).
A Quantitative Proteomic Analysis of Brassinosteroid-induced Protein Phosphorylation in Rice (Oryza sativa L.).
复制标题
水稻 (Oryza sativa L.) 中油菜素类固醇诱导的蛋白质磷酸化的定量蛋白质组学分析
DOI：
10.3389/fpls.2017.00514
复制
发表时间：
2017
影响因子：
5.6
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Hou Y;Qiu J;Wang Y;Li Z;Zhao J;Tong X;Lin H;Zhang J
油菜素类固醇（brassinosteroids，BR）是一类影响植物生长发育和胁迫反应的多羟基类固醇激素。BR信号转导在很大程度上依赖于蛋白磷酸化。通过采用无标记，MS（质谱）为基础的磷酸化蛋白质组学方法，我们在这里报告的最大的4,034个磷酸化位点的1,900磷蛋白从水稻幼苗和他们的动态响应BR。在BR处理期间，发现1，821种蛋白质，包括激酶、转录因子和BR和其他激素信号通路的核心组分，被差异磷酸化。Western印迹分析验证了这些蛋白质中的五个的差异磷酸化，这意味着基于MS的磷酸化蛋白质组学数据是稳健的。因此，赤霉素（GA）信号通路的去磷酸化可能是BR调控GA拮抗作用的重要机制，BR通过磷酸化调节纤维素合成而影响水稻的植株构型。
The group of polyhydroxysteroid phytohormones referred to as the brassinosteroids (BRs) is known to act on plant development and the stress response. BR signal transduction relies largely on protein phosphorylation. By employing a label-free, MS (Mass Spectrometry)-based phosphoproteomic approach, we report here the largest profiling of 4,034 phosphosites on 1,900 phosphoproteins from rice young seedlings and their dynamic response to BR. 1,821 proteins, including kinases, transcription factors and core components of BR and other hormone signaling pathways, were found to be differentially phosphorylated during the BR treatment. A Western blot analysis verified the differential phosphorylation of five of these proteins, implying that the MS-based phosphoproteomic data were robust. It is proposed that the dephosphorylation of gibberellin (GA) signaling components could represent an important mechanism for the BR-regulated antagonism to GA, and that BR influences the plant architecture of rice by regulating cellulose synthesis via phosphorylation.