Expression of proliferating cell nuclear antigen in bronchial epithelium after lung transplantation in the rat.
Expression of proliferating cell nuclear antigen in bronchial epithelium after lung transplantation in the rat.
复制标题
大鼠肺移植后支气管上皮增殖细胞核抗原的表达。
DOI：
10.4049/jimmunol.150.6.2494
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
H. Ayabe
中科院分区：
文献类型：
--
作者：
T. Nagayasu;T. Oka;T. Sawada;K. Tamura;T. Shirafuji;C. Ryu;H. Ayabe
背景
实验动物中的正常成熟气道上皮细胞具有非常缓慢的细胞更新和最小的增殖。本研究的目的是探讨增殖细胞核抗原（PCNA）的表达作为一个指标的支气管细胞增殖在布朗挪威刘易斯大鼠肺移植模型有或无免疫抑制。
方法
布朗挪威左肺移植到刘易斯受体。一些受体接受了高剂量的环孢素和FK 506治疗。Lewis-至-刘易斯供体-受体组合作为对照进行。在术后第3天和第5天切除肺。石蜡包埋切片，PCNA染色。将血管周围细胞浸润和支气管表面上皮中的PCNA阳性细胞计数与排斥反应的组织学分级进行比较。
结果
同种异体移植物支气管表面上皮细胞的PCNA指数（PCNA免疫染色细胞核的百分比）（3天为21.0% +/- 3.1%，5天为31.4% +/-9.8%，p < 0.05）显著高于同种异体移植物（3天为5.4% +/- 3.0%，5天为4.7% +/- 4.6%）。在排异肺的血管周围浸润中PCNA指数也更高（3天为23.9% +/- 3.7%，5天为29.1% +/- 6.6%）。然而，在环孢素和FK 506治疗组中，支气管表面上皮细胞的PCNA指数在第3天和第5天被抑制至小于5%。即使在移植后50天，在FK 506处理的移植物的支气管上皮中，PCNA阳性细胞也很少。
结论
同种移植物中的支气管上皮具有相对低的增殖率。在排斥反应中，同种异体移植物具有非常快速的细胞更新和增殖。上皮增生可能是免疫事件的结果，也可能促成这些事件的发病机制。
BACKGROUND
The normal, mature airway epithelium in experimental animals has a very slow cell turnover and minimal proliferation. The aim of this study was to investigate the expression of proliferating cell nuclear antigen (PCNA) as an index of bronchial cell proliferation in the Brown Norway to Lewis rat pulmonary allograft model with or without immunosuppression.
METHODS
Brown Norway left lungs were transplanted into Lewis recipients. Some recipients were treated with a high dose of cyclosporine and FK506. Lewis-to-Lewis donor-recipient combination was performed as a control. Lungs were excised on postoperative days 3 and 5. Routinely processed, paraffin-embedded sections were prepared and stained by PCNA. Counts of PCNA-positive cells in the perivascular cellular infiltrate and bronchial surface epithelium were compared with the histologic grade of rejection.
RESULTS
The PCNA index (percent of nuclei immunostaining for PCNA) in bronchial surface epithelium was significantly higher in allografts (21.0% +/- 3.1% at 3 days, 31.4% +/- 9.8 % at 5 days, p < 0.05) than in isografts (5.4% +/- 3.0% at 3 days, 4.7% +/- 4.6% at 5 days). The PCNA index was also greater in the perivascular infiltrates of rejecting lungs (23.9% +/- 3.7% at 3 days, 29.1% +/- 6.6% at 5 days). However, in the cyclosporine- and FK506-treated groups, the PCNA index in bronchial surface epithelium was suppressed to less than 5% at 3 and 5 days. Even at 50 days after transplantation, PCNA-positive cells were rare in bronchial epithelium of FK506-treated grafts.
CONCLUSIONS
Bronchial epithelium in isografts has a relatively low rate of proliferation. In rejection, allografts have a very rapid cell turnover and proliferation. Proliferating epithelium may be a consequence of immune events or it may contribute to the pathogenesis of those events.