Cellular tolerance to pulsed hyperthermia.
Cellular tolerance to pulsed hyperthermia.
复制标题
细胞对脉冲热疗的耐受性。
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
D. Palanker
中科院分区：
文献类型：
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作者：
D. Simanovskii;M. Mackanos;Afraaz R. Irani;C. O’Connell;C. Contag;H. A. Schwettman;D. Palanker
组织的短暂加热导致细胞应激或死亡在医学和生物学中是很常见的。在涉及轻度（低于70摄氏度）和长时间（分钟）加热的过程中，如高温肿瘤治疗，细胞对热应激的反应得到了相对较好的研究。然而，在高温和短时间暴露下，几乎没有关于细胞活力的数据，而对这方面知识的需求正在增长。推动这项研究的两个主要原因：(i)越来越多的激光治疗和涉及脉冲加热的外科手术程序，以及（ii）短时间暴露于高温下的细胞活力数据为理解导致热诱导细胞死亡的过程提供了独特的见解。我们设计了一种技术，并对持续时间为0.3 ~ 100 ms、峰值温度高达130℃的热脉冲下的细胞活力进行了研究。我们发现，在这个范围内，细胞死亡的阈值可以用阿累尼乌斯定律精确地近似计算，活化能为1 eV，这一数值明显低于基于多秒暴露的研究报告。
Transient heating of tissues leading to cellular stress or death is very common in medicine and biology. In procedures involving a mild (below 70 degrees C) and prolonged (minutes) heating, such as hyperthermal tumor therapy, the cellular response to thermal stress is relatively well studied. However, there is practically no data on cell viability at higher temperatures and shorter exposures, while the demand for this knowledge is growing. Two main reasons motivate this research: (i) a growing number of laser therapies and surgical procedures involving pulsed heating, and (ii) cellular viability data at short exposures to high temperatures provide a unique insight into the understanding of processes leading to thermally induced cellular death. We designed a technique and performed a study of cell viability under pulses of heat from 0.3 to 100 ms in duration with peak temperatures as high as 130 degrees C. We found that the threshold of cellular death in this range can be accurately approximated by the Arrhenius law with the activation energy of 1 eV, a significantly lower value than was reported in studies based on multisecond exposures.