CBL4-CIPK5 pathway confers salt but not drought and chilling tolerance by regulating ion homeostasis
CBL4-CIPK5 pathway confers salt but not drought and chilling tolerance by regulating ion homeostasis
复制标题
CBL4-CIPK5 通路通过调节离子稳态赋予盐耐受性，但不耐受干旱和寒冷
DOI：
10.1016/j.envexpbot.2020.104230
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发表时间：
2020-11-01
影响因子：
5.7
通讯作者：
Lu, Shaoyun
中科院分区：
文献类型：
--
作者：
Huang, Shilian;Chen, Miao;Lu, Shaoyun
钙调神经磷酸酶B样蛋白（CBL）和CBL相互作用蛋白激酶（CIPK）在植物非生物胁迫耐受性调控中起重要作用。CBL-CIPK途径是否调控狗牙根的非生物胁迫耐受性尚不清楚。在本研究中，CdtCBL 4和CdtCIPK 5从三倍体狗牙根进行了表征。CdtCBL 4和CdtCIPK 5转录物由脱水和盐诱导。此外，低温诱导CdtCIPK 5的转录，但抑制CdtCBL 4的转录;阿坝诱导CdtCIPK 5的转录，但对CdtCBL 4的转录没有影响。酵母双杂交和双分子荧光互补（BiFC）分析证实CdtCIPK 5与CdtCBL 4相互作用。结果表明，CdtCIPK 5和CdtCBL 4基因的过量表达提高了转基因水稻的耐盐性。CdtCIPK 5和CdtCBL 4过表达转基因株系在盐胁迫下具有较低的Na+积累和较高的K+/Na+比值。在CdtCIPK 5过表达植株中，OsNHX 3、OsHKT 1;1、OsHKT 1;4、OsHKT 1;5的转录水平高于野生型。CdtCBL 4-CdtCIPK 5途径可能通过上调OsNHX 3、OsHKT 1;1、OsHKT 1;4、OsHKT 1;5的表达来维持盐胁迫下Na+/K+的稳态，从而赋予植物耐盐性。
Calcineurin B-like protein (CBL) and CBL-interacting protein kinase (CIPK) play an important role in regulation on plant abiotic stress tolerance. It is unknown whether CBL-CIPK pathway regulates abiotic stress tolerance in bermudagrass. In the present study, CdtCBL4 and CdtCIPK5 from triploid bermudagrass were characterized. CdtCBL4 and CdtCIPK5 transcripts were induced by dehydration and salt. In addition, low temperature induced CdtCIPK5 transcript, but inhibited CdtCBL4 transcript; ABA induced CdtCIPK5 transcript, but had no influence on CdtCBL4 transcript. Yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assay demonstrated CdtCIPK5 interacted with CdtCBL4. Overexpression of CdtCIPK5 and CdtCBL4 improved salt tolerance in transgenic rice compared with wild type, based on measurements on relative water content (RWC), chlorophyll content and F-v/F-m. CdtCIPK5 and CdtCBL4 overexpressing transgenic lines had a lower Na+ accumulation and a higher K+/Na+ ratio under salt stress. The higher transcript levels of OsNHX3, OsHKT1;1, OsHKT1;4, OsHKT1;5 were observed in CdtCIPK5 overexpressing plants than in WT. It is suggested that CdtCBL4-CdtCIPK5 pathway confers salinity tolerance by upregulating OsNHX3, OsHKT1;1, OsHKT1;4, OsHKT1;5 expression for maintenance of Na+/K+ homeostasis during salt stress.