White lines in L-edge x-ray absorption spectra and their implications for anomalous diffraction studies of biological materials.
White lines in L-edge x-ray absorption spectra and their implications for anomalous diffraction studies of biological materials.
复制标题
L 边 X 射线吸收光谱中的白线及其对生物材料异常衍射研究的影响。
DOI：
10.1073/pnas.77.10.5884
复制
发表时间：
1980
影响因子：
11.1
通讯作者：
K. Hodgson
中科院分区：
文献类型：
--
作者：
R. Lye;J. C. Phillips;D. Kaplan;S. Doniach;K. Hodgson
我们已经测量了高分辨率的X射线吸收光谱的镧系元素（Ln）和重过渡金属配合物，显示突出的L2和L3吸收边附近的窄吸收峰。异常散射因子（f'和f”）在数学上与吸收截面相关，在吸收边的5-10 eV内其幅度具有相应的急剧变化。对f'和f”的变化幅度的计算表明，对于这些材料，可以预期显著的变化（在f'中的20个电子的数量级上）。异常散射因子的这些实质性变化可应用于从X射线衍射研究中获得大分子的结构信息。的变化幅度表明，异常散射技术是一个强大的手段，获得在单晶体中，在溶液中，和在生物膜中的大分子的结构特征。
We have measured high-resolution x-ray absorption spectra of lanthanide (Ln) and heavy transition metal complexes that display prominent narrow absorption peaks near the L2 and L3 absorption edges. The anomalous scattering factors (f' and f"), which are mathematically related to the absorption cross section, have correspondingly sharp changes in their magnitude within 5-10 eV of the absorption edge. Calculations of the magnitude of the change in f' and f" demonstrate that significant changes (on the order of 20 electrons in f') can be expected for these materials. These substantial changes in the anomalous scattering factors have applications to deriving structural information for macromolecules from x-ray diffraction studies. The magnitude of the changes indicate that the anomalous scattering technique is a powerful means of obtaining structural characteristics for macromolecules in single crystals, in solution, and in biological membranes.