Plasma cortisol determination: radioimmunoassay and competitive protein binding compared.
Plasma cortisol determination: radioimmunoassay and competitive protein binding compared.
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血浆皮质醇测定：放射免疫测定法和竞争性蛋白结合比较。
DOI：
10.1093/clinchem/20.4.411
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发表时间：
1974
影响因子：
9.3
通讯作者：
C. E. Pierce
中科院分区：
文献类型：
--
作者：
R. Farmer;C. E. Pierce
建立了一种简便的放射性配体法测定血浆和尿液中皮质醇的方法。以皮质醇-21-半琥珀酰-牛血清白蛋白为抗原，制备兔抗血清。标准曲线在0-1000 pg范围内呈线性。直接测定乙醇沉淀的血浆或尿液的小等分试样。在6个月期间，正常血浆池的56次独立测定的测定间变异性为：平均值224 µg/L;范围210-238 µg/L; CV 3.2%。在0800和1800小时之间从正常年轻男性获得的多个样品的测定中，放射免疫测定法（RIA）给出的值始终低于竞争性蛋白结合法（CPB）。根据41个单一样本的结果计算得出的关系式为RIA = 0.713 CPB + 0.59，表明RIA的正常范围应为35-180 µg/L，而不是通常引用的50-250 µg/L。虽然与11-脱氧皮质醇存在交叉反应性，但该检测方法明显上级CPB：它更特异，范围更宽（<8-400 µg/L），需要的技术人员时间更少。
A simple radioligand assay has been developed for measuring cortisol in plasma and urine. The antiserum was produced in rabbits by using cortisol-21-hemisuccinyl-bovine serum albumin as the antigen. The standard curve was linear from 0-1000 pg. A small aliquot of ethanol-precipitated plasma or urine is directly assayed. Interassay variability of 56 separate assays of a normal plasma pool during six months was: mean, 224 µg/liter; range, 210-238 µg/liter; CV, 3.2%. In assay of multiple samples obtained between 0800 and 1800 hours from normal young men, radioimmunoassay (RlA) gave consistently lower values than did competitive protein binding (CPB). The relationship, as calculated from results for 41 single samples, is expressed by the equation RIA = 0.713 CPB + 0.59, suggesting that the normal range for RIA should be 35-180 µg/liter rather than the 50-250 µg/liter usually cited. Although there is crossreactivity with 11-deoxycortisol, this assay is clearly superior to CPB: it is more specific, has a wider range (<8-400 µg/liter), and requires less technician time.