Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age
Vitrification of ICSI- and IVF-derived bovine blastocysts by minimum volume cooling procedure: effect of developmental stage and age
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DOI：
10.1016/j.theriogenology.2010.04.033
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发表时间：
2010-10-01
期刊：
影响因子：
2.8
通讯作者：
Hochi, S.
中科院分区：
文献类型：
--
作者：
Abdalla, H.;Shimoda, M.;Hochi, S.
目的是研究发育阶段（完全扩张或扩张囊胚）和/或年龄（在第7天或第8天收获）对来自卵胞浆内单精子注射（ICSI）或体外受精（IVF）的牛囊胚玻璃化冷冻后体外存活率的影响。ICSI衍生的完全扩张的囊胚（80%）的复温后存活率（24小时内囊胚腔再扩张）与IVF衍生的囊胚（88%）相似。然而，ICSI衍生的扩张囊胚在玻璃化冷冻程序中存活的能力（61%）低于IVF衍生的囊胚（85%; P < 0.05），尽管ICSI和IVF衍生的新鲜囊胚具有相似的质量。玻璃化冷冻前囊胚的年龄不影响ICSI衍生的（第7天和第8天胚胎分别为73%和59%）或IVF衍生的囊胚（第7天和第8天胚胎均为86%）的低温耐受性。在保温培养2 - 4 h后，玻璃化冷冻后存活的ICSI衍生的囊胚比IVF衍生的囊胚含有更高比例的死细胞（5-13% vs. 2-4%; P < 0.05），但这些比例与新鲜对照胚胎的比例没有差异。仅在IVF衍生的第8天囊胚中，玻璃化冷冻对囊胚在培养72小时内孵化的能力有不利影响（玻璃化冷冻组和新鲜对照组分别为41%和70%）。总之，如果在玻璃化冷冻前将ICSI和IVF衍生的牛囊胚培养至完全扩增阶段，则玻璃化冷冻程序中存活的囊胚比例相似，第7天与第8天收获的胚胎之间无显著差异。(C)2010年爱思唯尔公司All rights reserved.
The objective was to investigate the effects of developmental stage (fully-expanded or expanding blastocysts) and/or age (harvested on Days 7 or 8) on post-vitrification in vitro survival of bovine blastocysts derived from intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF). Post-warming survival (re-expansion of blastocoele within 24 h) of ICSI-derived fully-expanded blastocysts (80%) was similar to that of their IVF-derived counterparts (88%). However, the ability of ICSI-derived expanding blastocysts to survive vitrification procedures (61%) was lower than that of IVF-derived blastocysts (85%; P < 0.05), although the ICSI- and IVF-derived fresh blastocysts were of similar quality. The age of the blastocysts before vitrification did not affect cryotolerance for either ICSI-derived (73 and 59% for Days 7 and 8 embryos, respectively) or IVF-derived blastocysts (86% for both Days 7 and 8 embryos). At 24 h of post-warming culture, ICSI-derived blastocysts surviving vitrification contained a higher proportion of dead cells than their IVF-derived counterparts (5-13% vs. 2-4%; P < 0.05), but these proportions were not different from those of fresh control embryos. There was an adverse effect of vitrification on the ability of blastocysts to hatch within 72 h of culture only in IVF-derived Day 8 blastocysts (41 and 70% in vitrified and fresh control groups, respectively). In conclusion, the proportion of blastocysts that survived vitrification procedures was similar for ICSI- and IVF-derived bovine blastocysts if the former were cultured to the fully-expanded stage prior to vitrification, with no significant difference between embryos harvested on Day 7 versus Day 8. (C) 2010 Elsevier Inc. All rights reserved.