BASE-BASE MISMATCHES - THERMODYNAMICS OF DOUBLE HELIX FORMATION FOR DCA3XA3G + DCT3YT3G (X, Y = A,C,G,T)
BASE-BASE MISMATCHES - THERMODYNAMICS OF DOUBLE HELIX FORMATION FOR DCA3XA3G + DCT3YT3G (X, Y = A,C,G,T)
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DOI：
10.1093/nar/13.13.4811
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发表时间：
1985-01-01
影响因子：
14.9
通讯作者：
MARTIN, FH
中科院分区：
文献类型：
--
作者：
ABOULELA, F;KOH, D;MARTIN, FH
测定了dCA 3XA 3G·dCT 3 YT 3G序列的16个双螺旋的双链形成热力学参数，每个碱基A、C、G和T位于标记为X和Y的位置。结果进行了分析，在最近的邻居，并与RNA二级结构的热力学参数进行比较。在室温下， - A-A-T 更稳定 - A-A-U·-U·- 并且在稳定性方面类似于 -A-C-T·-G·- -C-A-G·-T·- -A-G-T·-C·- -G-A--C·-T·- ; - T-T·-A· 和 -T-A-A·-T· 最不稳定。在较高温度下，含有G·C碱基对的序列比只含有A·T的序列更稳定。所有含有错配的分子相对于仅具有沃森-克里克配对的那些分子是不稳定的，但是存在广泛的不稳定。在室温下，最稳定的错配是含有鸟嘌呤的错配（G·T，G·C，G·A）;最不稳定的错配是含有胞嘧啶的错配（C·A，C·C）。在较高温度下，嘧啶-嘧啶错配变得最不稳定。
Thermodynamic parameters for double strand formation have been measured for the sixteen double helices of the sequence dCA3XA3G·dCT3YT3G, with each of the bases A, C, G and T at the positions labelled X and Y. The results are analyzed in terms of nearest-neighbors and are compared with thermodynamic parameters for RNA secondary structure. At room temperature the sequence -A-A--T•-T•- is more stable than -A-A--U•-U•- and is similar in stability to -A-C--T•-G•- , -C-A--G•-T•- , -A-G--T•-C•- , -G-A--C•-T•- ; -A-T--T•-A•- and -T-A--A•-T•- are least stable. At higher temperatures the sequences containing a G·C base pair become more stable than those containing only A·T. All molecules containing mismatches are destabilized with respect to those with only Watson-Crick pairing, but there is a wide range of destabilization. At room temperature the most stable mismatches are those containing guanine (G·T, G·C, G·A); the least stable contain cytosine (C·A, C·C). At higher temperatures pyrimidine-pyrimidine mismatches become the least stable.