Structure of the cortical cytoskeleton in mammalian outer hair cells.
Structure of the cortical cytoskeleton in mammalian outer hair cells.
复制标题
哺乳动物外毛细胞皮质细胞骨架的结构。
作者：
M. Holley;F. Kalinec;B. Kachar
外毛细胞中的皮质细胞骨架晶格是一种基于肌动蛋白的二维结构，它可以被罗丹明/鬼臼毒素标记，并被脱氧核糖核酸酶I酶破坏。来自薄片、冰冻蚀刻和负染制备的结构信息表明，它是基于两种类型的细丝，这两种类型的细丝形成了环状细丝的交联格。交联物的长度为70-80 nm。对圆周细丝之间的间距的测量表明，晶格的圆周方向比纵向更坚硬。对周向细丝取向的分析表明，它由长达10微米的离散区域组成2。区域之间的相对运动可以使细胞形状发生实质性变化，而不破坏晶格的单位结构，从而使细胞皮质保持对高频变形的弹性反应。
The cortical cytoskeletal lattice in outer hair cells is a two-dimensional actin-based structure, which can be labelled with rhodamine/phalloidin and disrupted by the enzyme deoxyribonuclease I. Structural information from thin sectioned, freeze-etched and negatively stained preparations shows that it is based upon two types of filament that form a cross-linked lattice of circumferential filaments. The cross-links are 70-80 nm long. Measurements of the spacing between circumferential filaments suggest that the lattice is stiffer circumferentially than it is longitudinally. Analysis of the orientation of circumferential filaments shows that it is composed of discrete domains of up to 10 microns 2. Relative movements between domains could allow substantial changes of cell shape without disrupting the unit structure of the lattice, thus allowing the cell cortex to retain its elastic responses to high-frequency deformations.