Plant cell graft chimeras obtained by co-culture of isolated protoplasts
Plant cell graft chimeras obtained by co-culture of isolated protoplasts
复制标题
DOI：
10.1007/bf01276789
复制
发表时间：
1987-02
期刊：
影响因子：
2.9
通讯作者：
H. Binding;D. Witt;J. Monzer;G. Mordhorst;R. Kollmann
中科院分区：
文献类型：
--
作者：
H. Binding;D. Witt;J. Monzer;G. Mordhorst;R. Kollmann
携带质体标记和二单倍体的龙葵克隆的原生质体。 tuberosumclone在液体培养基中共培养以产生嵌合细胞系。根据形态学和细胞学标准，从这些品系再生的小植株被鉴定为周周、扇形和节状异种嵌合体。所有 17 个品系的植物均在组织培养条件下生长至开花，有些还在温室中生长。这两个物种的细胞通过次生胞间连丝相互连接。
Protoplasts ofSolanum nigrumclones carrying plastid markers and a dihaploidS. tuberosumclone were co-cultured in liquid medium to produce chimaerical cell lines. Plantlets regenerated from these lines were identified as periclinal, sectorial and mericlinal heterospecific chimaeras by morphological and cytological criteria. Plants of all 17 lines were grown to flowering under tissue culture conditions and some also in the greenhouse. Cells of the 2 species were interconnected by secondary plasmodesmata.