Dimethylselenide Demethylation Is an Adaptive Response to Selenium Deprivation in the Archaeon Methanococcus voltae
Dimethylselenide Demethylation Is an Adaptive Response to Selenium Deprivation in the Archaeon Methanococcus voltae
复制标题
二甲基硒化物去甲基化是古细菌伏氏甲烷球菌对缺硒的适应性反应
作者：
Ulf M. Niess;A. Klein
摘要古菌甲烷球菌需要硒来维持最佳生长。发现了一个最可能参与二甲基硒化物去甲基化的基因组，其表达在硒剥夺后被诱导。该操纵子包含一个类咕啉蛋白的开放阅读框和两个推定的甲基转移酶。它表明，二甲基硒化物的硒耗尽的生长培养基中的添加缓解硒的缺乏，所示的抑制的转录单位编码的无硒氢化酶，这是正常情况下只有在硒剥夺活性的启动子。类咕啉蛋白或两个甲基转移酶基因之一的敲除突变体在二甲基硒化物的存在下没有表现出对氢化酶启动子的抑制。另一个甲基转移酶基因的突变没有影响。两个有效的突变体的生长速率降低相比，野生型细胞在硒限制培养基中的二甲基硒化物的存在下。
ABSTRACT The archaeon Methanococcus voltae needs selenium for optimal growth. A gene group most likely involved in the demethylation of dimethylselenide was discovered, the expression of which is induced upon selenium deprivation. The operon comprises open reading frames for a corrinoid protein and two putative methyltransferases. It is shown that the addition of dimethylselenide to selenium-depleted growth medium relieves the lack of selenium, as indicated by the repression of a promoter of a transcription unit encoding selenium-free hydrogenases which is normally active only upon selenium deprivation. Knockout mutants of the corrinoid protein or one of the two methyltransferase genes did not show repression of the hydrogenase promoter in the presence of dimethylselenide. The mutation of the other methyltransferase gene had no effect. Growth rates of the two effective mutants were reduced compared to wild-type cells in selenium-limited medium in the presence of dimethylselenide.
影响因子：
2.9
作者：
CHOMCZYNSKI, P
通讯作者：
CHOMCZYNSKI, P
DOI：
10.1073/pnas.94.6.2626
发表时间：
1997-03-18
影响因子：
11.1
作者：
Metcalf, WW;Zhang, JK;Wolfe, RS
通讯作者：
Wolfe, RS