Fsp27 plays a crucial role in muscle performance.
Fsp27 plays a crucial role in muscle performance.
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DOI：
10.1152/ajpendo.00255.2021
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发表时间：
2022-02
期刊：
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通讯作者：
Mark Slayton;Bijinu Balakrishnan;Abhishek Gupta;Scott Jobe;Ishika Puri;Savannah Neely;Y. Tamori;D. Russ;Gozde Yildrim;S. Yakar;V. Sharma;V. Puri
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作者：
Mark Slayton;Bijinu Balakrishnan;Abhishek Gupta;Scott Jobe;Ishika Puri;Savannah Neely;Y. Tamori;D. Russ;Gozde Yildrim;S. Yakar;V. Sharma;V. Puri
Fsp27 先前被鉴定为脂肪细胞中的脂滴相关蛋白。各种研究表明，它在调节脂肪组织和肝脏的脂质稳态中发挥作用。然而，它在肌肉中的功能以及脂肪的积累和代谢仍然完全未知。我们目前的研究确定了 Fsp27 在肌肉表现中的新作用。在这里，我们证明与野生型对照相比，Fsp27-/- 和 Fsp27+/- 小鼠（无论雄性还是雌性）的肌肉耐力和运动能力严重受损。所有进食或禁食组以及运动前后的肝脏和肌肉糖原储存相似。 Fsp27-/- 和 Fsp27+/- 小鼠的肌肉性能下降与肌肉中脂肪含量的严重下降有关。此外，杂合 Fsp27+/- 小鼠的结果表明，Fsp27 单倍体不足会损害雄性和雌性的肌肉性能。总之，我们的生理学研究结果表明，Fsp27 在肌肉脂肪储存、肌肉耐力和肌肉力量中发挥着关键作用。
Fsp27 was previously identified as a lipid droplet-associated protein in adipocytes. Various studies have shown that it plays a role in the regulation of lipid homeostasis in adipose tissue and liver. However, its function in muscle, which also accumulate and metabolize fat, remains completely unknown. Our present study identifies a novel role of Fsp27 in muscle performance. Here we demonstrate that Fsp27-/- and Fsp27+/- mice, both males and females, had severely impaired muscle endurance and exercise capacity compared to wild-type controls. Liver and muscle glycogen stores were similar amongst all groups fed or fasted, and before or after exercise. Reduced muscle performance in Fsp27-/- and Fsp27+/- mice was associated with severely decreased fat content in the muscle. Furthermore, results in heterozygous Fsp27+/- mice indicate that Fsp27 haploinsufficiency undermines muscle performance in both males and females. In sum, our physiological findings reveal that Fsp27 plays a critical role in muscular fat storage, muscle endurance, and muscle strength.