Failure of DNA barcoding in discriminating Calligonum species
Failure of DNA barcoding in discriminating Calligonum species
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DNA 条形码在区分沙拐枣属物种中的失败
DOI：
10.1111/njb.00423
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发表时间：
2014-08-01
影响因子：
0.9
通讯作者：
Lv, Guang-Hui
中科院分区：
文献类型：
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作者：
Li, Yan;Feng, Ying;Lv, Guang-Hui
研究了4个陆地植物DNA条形码标记（rbcL、matK、ITS和trnLF区域）在沙拐枣属植物鉴定中的有效性。用通用引物获得rbcL、matK和trnLF的高质量序列，而ITS序列质量较差。RbcL和matK具有高度保守性，不能区分物种。当rbcL、matK和trnLF联合使用时，物种分辨率可达6.25%。低序列变异导致解析不佳的树拓扑结构。在16个采样的物种中，只有三个被恢复为一个单系群。我们的研究结果表明，虽然DNA条形码是一个重要的工具，物种鉴定，它不能区分沙拐枣属物种。需要进一步的研究来开发能够区分这种分类复杂且最近分歧的属中的物种的标记。
We tested the effectiveness of four DNA barcoding markers (rbcL, matK, ITS and trnLF region) for land plants in identifying Calligonum species. High quality sequences were obtained for rbcL, matK and trnLF with the universal primers whereas ITS sequences were of poor quality. RbcL and matK were highly conservative and failed in species discrimination. When rbcL, matK and trnLF were combined, the species resolution was up to 6.25%. Low sequence variation resulted in poorly resolved tree topologies. Among the sixteen sampled species, only three were recovered as a monophyletic group. Our results show that although DNA barcoding is an important tool for species identification, it fails in discriminating Calligonum species. Further research will be needed to develop markers capable to discriminate species in this taxonomy complicated and recently diverged genus.