The full length coding sequence of rat liver androsterone UDP-glucuronyltransferase cDNA and comparison with other members of this gene family.
The full length coding sequence of rat liver androsterone UDP-glucuronyltransferase cDNA and comparison with other members of this gene family.
复制标题
大鼠肝脏雄酮UDP-葡萄糖醛酸转移酶cDNA的全长编码序列以及与该基因家族其他成员的比较。
作者：
M. Jackson;B. Burchell
已使用抗UDP-葡糖醛酸转移酶（UDPGT）抗体从表达载体λ gt 11中的大鼠肝cDNA文库中分离编码肝UDP-葡糖醛酸转移酶（UDPGT）的克隆cDNA。通过对15个UDPGT cDNA克隆进行测序，已经鉴定出四种不同的mRNA。这四类cDNA的序列被确定为85-95%同源。从每个类别的cDNA中分离限制性片段并用作类别特异性探针。将这些探针与从正常和遗传缺陷Wistar大鼠的肝脏制备的总RNA的北方印迹杂交，鉴定了具有雄酮UDPGT的第4类cDNA。翻译的cDNA序列的克隆rlug 23，类4的最长成员，允许确定的完整的氨基酸序列的雄甾酮UDPGT。
Cloned cDNAs coding for hepatic UDP-glucuronyltransferase (UDPGT) have been isolated from a rat liver cDNA library in the expression vector bacteriophage lambda gt11 using anti-UDPGT antibodies. Four different mRNAs have been identified by sequencing of 15 UDPGT cDNA clones. The sequences of the four classes of cDNA were determined to be 85-95% homologous. Restriction fragments were isolated from the cDNA in each class and used as class specific probes. Hybridisation of these probes to northern blots of total RNA prepared from the livers of normal and genetically deficient Wistar rats identified the cDNA in class 4 with androsterone UDPGT. Translation of the cDNA sequence of clone rlug 23, the longest member of class 4, allowed determination of the complete amino acid sequence of androsterone UDPGT.