Identification of putative transcription factor binding sites in rodent selenoprotein W promoter
Identification of putative transcription factor binding sites in rodent selenoprotein W promoter
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DOI：
10.1016/j.jinorgbio.2004.06.003
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发表时间：
2004-09-01
影响因子：
3.9
通讯作者：
Whanger, PD
中科院分区：
文献类型：
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作者：
Amantana, A;Vorachek, WR;Whanger, PD
为了了解硒蛋白W（SeW）基因的转录调控，我们使用体外结合试验来鉴定可能参与SeW基因转录调控的转录因子。使用蛋白质从大鼠C6（神经胶质）细胞核提取物，寡核苷酸含有推定的监管元件在SeW启动子和抗体，我们观察到特异性蛋白1（Sp1）转录因子结合到Sp1的共识序列在SeW启动子以及金属反应元件（MRE）。虽然竞争分析显示在TFII-1位点的特异性结合，但使用抗TFII-1抗体的超位移分析未产生任何超位移条带。因此，SeW基因可能是Sp1的靶标，Sp1与SeW启动子的各种调控序列的结合可以激活或抑制SeW的转录。还检测了MRE、GRE、AP-1和LF-A1位点，但未获得特异性结合的证据，表明与未标记探针缺乏竞争。(C)2004年爱思唯尔公司All rights reserved.
To understand transcriptional regulation of the selenoprotein W (SeW) gene, we used in vitro binding assays to identify transcription factors that may be involved in the transcriptional regulation of the SeW gene. Using protein from rat C6 (glial) cell nuclear extracts, oligonucleotides containing putative regulatory elements in the SeW promoter and antibodies, we observed that specificity protein 1(Sp1) transcription factor binds to the Sp1 consensus sequence in the SeW promoter as well as to the metal response element (MRE). Although competition analysis showed specific binding at the TFII-1 site, super-shift analysis using anti-TFII-1 antibody did not yield any super-shifted band. Therefore, the SeW gene may be a target for Sp1 whose binding to various regulatory sequences of the SeW promoter may activate or repress the transcription of SeW. The MRE, GRE, AP-1 and LF-A1 sites were also tested but no evidence was obtained for specific binding as indicated by lack of competition with unlabeled probes. (C) 2004 Elsevier Inc. All rights reserved.