The manifold phospholipases A of Legionella pneumophila -: Identification, export, regulation, and their link to bacterial virulence
The manifold phospholipases A of Legionella pneumophila -: Identification, export, regulation, and their link to bacterial virulence
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DOI：
10.1016/j.ijmm.2007.11.004
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发表时间：
2008-04-01
影响因子：
4.1
通讯作者：
Flieger, Antje
中科院分区：
文献类型：
--
作者：
Banerji, Sangeeta;Aurass, Philipp;Flieger, Antje
细胞内肺病原体嗜肺军团菌表达属于至少三个酶家族的分泌型和细胞相关型磷脂酶A（PLA）和溶血磷脂酶A（LPLA）活性。第一个家族由三种分泌的PLA和LPLA活性组成，展示氨基酸特征基序“GDSL”; PlaA、PlaC和PlaD。第二组包含细胞相关的和非常有效的PLA/LPLA，PlaB。第三组，马铃薯糖蛋白样蛋白，包括II成员。一种patatin样蛋白PatA/VipD在酵母中表达时显示LPLA和PLA活性并干扰囊泡运输，因此可能参与细胞内感染过程。同样，前两个磷脂酶家族的成员在细菌毒力中起作用，因为磷脂酶是重要的毒力因子，已显示其促进细菌存活、扩散和宿主细胞修饰/损伤。GDSL酶PlaA解毒溶细胞溶血磷脂，PlaB显示接触依赖性溶血活性。PlaC酰化胆固醇，一种存在于真核宿主中但不存在于细菌中的脂质。许多L。嗜肺菌PLA通过参与毒力因子输出的II型Lsp或IVB型Dot/Icm分泌系统输出。此外，脂解活性的调节依赖于转录调节因子LetA/S和RpoS，诱导毒力性状的表达，以及转录后激活因子如锌金属蛋白酶ProA。(c)2007年，Elsevier GmbH。All rights reserved.
The intracellular lung pathogen Legionella pneumophila expresses secreted and cell-associated phospholipase A (PLA) and lysophospholipase A (LPLA) activities belonging to at least three enzyme families. The first family consists of three secreted PLA and LPLA activities displaying the amino acid signature motif 'GDSL'; PlaA, PlaC and PlaD. The second group contains the cell-associated and very potent PLA/LPLA, PlaB. The third group, the patatin-like proteins, comprises I I members. One patatin-like protein, PatA/VipD, shows LPLA and PLA activities and interferes with vesicular trafficking when expressed in yeast and therefore is possibly involved in the intracellular infection process. Likewise, members of the first two phospholipase families have roles in bacterial virulence because phospholipases are important virulence factors that have been shown to promote bacterial survival, spread and host cell modification/damage. The GDSL enzyme PlaA detoxifies cytolytic lysophospholipids, and PlaB shows contact-dependent haemolytic activity. PlaC acylates cholesterol, a lipid present in eukaryotic hosts but not in the bacterium. Many of the L. pneumophila PLAs are exported by the type II Lsp or the type IVB Dot/Icm secretion systems involved in virulence factor export. Moreover, the regulation of lipolytic activities depends on the transcriptional regulators LetA/S and RpoS, inducing the expression of virulence traits, and on posttranscriptional activators like the zinc metalloprotease ProA. (c) 2007 Elsevier GmbH. All rights reserved.