Rice SERK1 gene positively regulates somatic embryogenesis of cultured cell and host defense response against fungal infection
Rice SERK1 gene positively regulates somatic embryogenesis of cultured cell and host defense response against fungal infection
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DOI：
10.1007/s00425-005-1534-4
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发表时间：
2005-09-01
期刊：
影响因子：
4.3
通讯作者：
Yang, Y
中科院分区：
文献类型：
--
作者：
Hu, H;Xiong, L;Yang, Y
本文报道了水稻体细胞胚胎发生受体样激酶（OsSERK 1）基因的分离和鉴定。OsSERK 1基因属于水稻类受体激酶基因的一个小亚家族，与已报道的植物SERK基因具有高度保守的基因结构和广泛的序列同源性。虽然该基因在水稻各器官/组织中都有基础水平的表达，但在水稻体细胞胚胎发生过程中的愈伤组织中表达量较高。利用RNA干扰技术抑制OsSERK 1基因在转基因愈伤组织中的表达，可显著降低粳稻中花11的再生率（从72%降至14%）。而过量表达OsSERK 1则提高了不定芽再生率（从72%提高到86%）。有趣的是，OsSERK 1是显着激活稻瘟病菌，特别是在不相容的相互作用，并与宿主细胞死亡的Sekigushi病变模拟突变体。该基因也可以被防御信号分子（例如水杨酸、茉莉酸和脱落酸）诱导。此外，OsSERK 1在两个水稻品种中的组成型过表达导致了寄主对稻瘟病菌抗性的增强。我们的数据表明，OsSERK 1可能部分介导防御信号转导除了其在体细胞胚胎发生的基本作用。
Here we report on the isolation and characterization of a somatic embryogenesis receptor-like kinase (OsSERK1) gene in rice (Oryza sativa). The OsSERK1 gene belongs to a small subfamily of receptor-like kinase genes in rice and shares a highly conserved gene structure and extensive sequence homology with previously reported plant SERK genes. Though it has a basal level of expression in various rice organs/tissues, as high expression level was detected in rice callus during somatic embryogenesis. Suppression of OsSERK1 expression in transgenic calli by RNA interference resulted in a significant reduction of shoot regeneration rate (from 72% to 14% in the japonica rice Zhonghua11). Overexpression of OsSERK1, however, increased the shoot regeneration rate (from 72% to 86%). Interestingly, OsSERK1 is significantly activated by the rice blast fungus, particularly during the incompatible interaction, and is associated with host cell death in Sekigushi lesion mimic mutants. This gene is also inducible by defense signaling molecules such as salicylic acid, jasmonic acid, and abscisic acid. Furthermore, constitutive overexpression of OsSERK1 in two rice cultivars led to an increase in host resistance to the blast fungus. Our data suggest that OsSERK1 may partially mediate defense signal transduction in addition to its basic role in somatic embryogenesis.