TLR9 and BAFF: Their expression in patients with IgA nephropathy
TLR9 and BAFF: Their expression in patients with IgA nephropathy
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TLR9 和 BAFF：它们在 IgA 肾病患者中的表达
DOI：
10.3892/mmr.2014.2359
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发表时间：
2014-09-01
影响因子：
3.4
通讯作者：
Peng, Youming
中科院分区：
文献类型：
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作者：
Li, Weiwei;Peng, Xiaofei;Peng, Youming
自1968年首次描述以来，免疫球蛋白（IG）A肾病（IgAN）已成为全球最常见的原发性肾小球疾病。许多报告显示Toll样受体9（TLR 9）和B细胞活化因子（BAFF）可能与IgAN相关;然而，尚未提供足够的证据。在本研究中，BAFF的血清水平以及TLR 9的mRNA和蛋白水平在外周血单核细胞（PBMC）进行了评估。采用定量聚合酶链反应（PCR）检测PBMC中TLR 9 mRNA的表达，Western blot检测PBMC中TLR 9蛋白的表达。采用特异性ELISA法检测血清BAFF和IgA 1水平。IgA肾病患者血清BAFF、IgA 1水平及外周血单个核细胞TLR 9 mRNA和蛋白水平均显著高于轻度肾小球异常患者（P
Since it was first described in 1968, immunoglobulin (Ig)A nephropathy (IgAN) has become the most commonly diagnosed form of primary glomerular disease worldwide. A number of reports have shown that toll-like receptor 9 (TLR9) and B-cell activating factor (BAFF) may be associated with IgAN; however, sufficient evidence has not yet to be delivered. In the present study, serum levels of BAFF as well as TLR9 mRNA and protein levels in peripheral blood mononuclear cells (PBMCs) were assessed. Expression of TLR9 mRNA in PBMCs was examined by quantitative polymerase chain reaction and the TLR9 protein was determined by western blot analysis. The levels of serum BAFF and IgA1 were determined by specific ELISA. Serum levels of BAFF and IgA1 as well as levels of TLR9 mRNA and protein in PMBCs were significantly higher in patients with IgAN compared with patients with minimal glomerular abnormalities (P