The synthesis of hydroxyapatite with different crystallinities by controlling the concentration of recombinant CEMP1 for biological application
The synthesis of hydroxyapatite with different crystallinities by controlling the concentration of recombinant CEMP1 for biological application
复制标题
控制重组CEMP1浓度合成不同结晶度羟基磷灰石的生物应用
DOI：
10.1016/j.msec.2015.10.029
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发表时间：
2016-02-01
影响因子：
7.9
通讯作者：
Sun, Weibin
中科院分区：
文献类型：
--
作者：
Chen, Xiaofeng;Liu, Yu;Sun, Weibin
牙骨质蛋白1（CEMP 1）是牙骨质特异性的，它在牙骨质矿化的性质和结构中起作用。本研究旨在验证CEMP 1在靶向位点积累时具有引导有序HA形成的能力这一假设。采用仿生方法制备了蛋白质诱导的晶体。在48小时期间监测CEMP 1浓度（0-100 μ g/ml）对磷酸钙沉淀速率的影响，同时利用透射电子显微镜（TEM）、选区电子衍射（SAED）、X射线衍射（XRD）和傅里叶变换红外光谱（FTIR）评估形成的矿物相。结果表明，重组牙骨质蛋白1（rhCEMP 1）对晶体的成核和生长有影响。在较低的rhCEMP 1浓度（0-50 μ g/ml）下，发生有限的矿物质形成，并且仅发现小束的HA晶体，然而，在100 μ g/ml的rhCEMP 1下，观察到主要是有组织的线性针状HA晶体。红外光谱显示HA的特征谱带出现在1106 cm（-1）、557 cm（-1）和598 cm（-1）处。因此，增加rhCEMP 1的浓度可以导致HA晶体在体外形成有序束。(C)2015 Elsevier B. V.版权所有。
Cementum protein 1 (CEMP1) has been reported to be cementum specific and it plays a role in the properties and structure of cementum mineralization. This study was carried out to test the hypothesis that, CEMP1 had the capacity to guide ordered HA formation as it accumulated at targeting site. Crystals induced by protein were prepared by biomimetic method. The effects of CEMP1 concentrations (0-100 mu g/ml) on the rate of calcium phosphate precipitation was monitored during 48 h, while the formed mineral phases were assessed utilizing transmission electron microscope (TEM), selected area electron diffraction (SAED), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The results showed that recombinant cementum protein 1 (rhCEMP1) affected the crystal nucleation and growth. At lower rhCEMP1 concentrations (0-50 mu g/ml), limited mineral formation occurred and only small bundles of HA crystals were found. However, with 100 mu g/ml of rhCEMP1, a predominance of organized linear needle-like HA crystals was observed. FTIR revealed the characteristic bands of HA appearing at 1106 cm(-1), 557 cm(-1), and 598 cm(-1). Therefore, increasing the concentration of rhCEMP1 can lead to the formation of ordered bundles of HA crystals in vitro. (C) 2015 Elsevier B.V. All rights reserved.