Photoinduced formation of flavin radicals in BLUF domains lacking the central glutamine
Photoinduced formation of flavin radicals in BLUF domains lacking the central glutamine
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DOI：
10.1111/febs.13297
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发表时间：
2015-08-01
期刊：
影响因子：
5.4
通讯作者：
Mathes, Tilo
中科院分区：
文献类型：
--
作者：
Fudim, Roman;Mehlhorn, Jennifer;Mathes, Tilo
使用FAD （BLUFs）的蓝光受体通过光诱导黄素发色团和保守的谷氨酰胺侧链之间的氢键重排来促进蓝光诱导的信号转导。本文研究了聚胞藻（Synechocystis sp.）中去除了谷氨酰胺侧链的blf结构域Slr1694的光化学性质。没有谷氨酰胺，没有形成红移信号状态，但光诱导的质子耦合电子在蛋白质和黄素之间转移，与野生型蛋白质相似。然而，中性黄素半醌-酪氨酸自由基对的寿命大大延长
Blue light receptors using FAD (BLUFs) facilitate blue light-induced signal transduction via light-induced rearrangement of hydrogen bonds between the flavin chromophore and a conserved glutamine side chain. Here, we investigated the photochemistry of the BLUF domain Slr1694 from Synechocystis sp. in which the glutamine side chain was removed. Without the glutamine, no red-shifted signaling state is formed, but light-induced proton-coupled electron transfer between protein and flavin takes place similarly as for the wild-type protein. However, the lifetime of the neutral flavin semiquinone-tyrosyl radical pair is greatly prolonged from